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Anti-Adeno-associated virus 8, clone HL2383, Cat. No. MABF2306, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 8 and has been tested for use in Dot Blot, ELISA and Neutralizing Applications.
More>>Anti-Adeno-associated virus 8, clone HL2383, Cat. No. MABF2306, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 8 and has been tested for use in Dot Blot, ELISA and Neutralizing Applications. Less<<
Adenoviruses are large (65-80 nm), non-enveloped viruses containing a dsDNA genome. They typically cause mild infections involving the upper or lower respiratory tract, gastrointestinal (GI) tract, or conjunctiva. However rare manifestations of adenovirus infections may include hemorrhagic cystitis, hepatitis, hemorrhagic colitis, pancreatitis, nephritis, or encephalitis. They also are used gene delivery vectors. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. The principal component of adenovirus is the homotrimeric hexon and there are 240 on the faces and edges of the capsid with the pentons consisting of the penton bases and extended fibers on the 12 fivefold apices. The size of the hexon molecule can vary with the serotype. The base of each hexon molecule has one loop and two eight-stranded jelly rolls that are important for interacting with neighboring capsomeres. Other so-called minor components: IIIa, VI, VIII and IX are also associated with the capsid. There are six other structural components situated in the virus core, of which five are associated with the double stranded DNA genome and the remaining component is the 23K virion protease that plays a vital role in the assembly of the virion. AAV8 shown to be suitable for use hepatic cell transduction and can serve as a useful vector for studies involving the hepatic tissue. Clone HL2383 generated against AAV8 capsid, binds to AAV8, but also displays cross-reactivity with AAV3B. It can also neutralize infection in cells. (Ref.: Russell, WC et al. (2009). J. Gen. Virol. 90(1); 1-20; Tseng, Y-S., et al. (2016). J. Virol. Methods. 236; 105-110).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Applications
Application
Anti-Adeno-associated virus 8, clone HL2383, Cat. No. MABF2306, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 8 and has been tested for use in Dot Blot, ELISA and Neutralizing Applications.
Key Applications
Dot Blot
ELISA
Neutralizing
Application Notes
Dot Blot Analysis: A representative lot detected Adeno-associated virus 8 in Dot Blot applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
Neutralizing Analysis: A representative lot neutralized Adeno-associated virus infection in cells (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
ELISA Analysis: A representative lot detected Adeno-associated virus 8 in ELISA applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
Clone HL2383 is a mouse monoclonal antibody that detects AAV8 capsid protein. Also displays cross-reactivity with AAV3B capsid protein.
Isotype
IgG3κ
Species Reactivity
Virus
Species Reactivity Note
Virus (Adenovirus)
Antibody Type
Monoclonal Antibody
Purification Method
Protein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot with AAV8 and AAV9 virus like particles .
Dot Blot Analysis: Various dilutions of AAV8 and AAV9 virus like particles were probed with Adenovirus 8. This antibody specifically detects AAV8, but not AAV9.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Adeno-associated viruses (AAVs) are promising viral vectors for therapeutic gene delivery, and the approval of an AAV1 vector for the treatment of lipoprotein lipase deficiency has heralded a new and exciting era for this system. However, preclinical and clinical studies show that neutralization from pre-existing antibodies is detrimental for medical application and this hurdle must be overcome before full clinical realization can be achieved. Thus the binding sites for capsid antibodies must be identified and eliminated through capsid engineering. Towards this goal and to recapitulate patient polyclonal responses, a panel of six new mouse monoclonal antibodies (MAbs) has been generated against AAV8 and AAV9 capsids, two vectors being developed for therapeutic application. Native (capsid) dot blot assays confirmed the specificity of these antibodies for their parental serotypes, with the exception of one MAb, HL2372, selected to cross-react against both capsids. Furthermore, in vitro assays showed that these MAbs are capable of neutralizing virus infection. These MAbs will be utilized for structural mapping of antigenic footprints on their respective capsids to inform development of the next generation of rAAV vectors capable of evading antibody neutralization while retaining parental tropism.
