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14-276 Akt1/PKBα Protein, active, 15 µg

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14-276
15 µg  Also available in 250μg size, please inquire for pricing and availability.
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      Übersicht

      Replacement Information
      Description
      Catalogue Number14-276
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAkt1/PKBα Protein, active, 15 µg
      OverviewRecombinant full-length human Akt1, containing N-terminal His6 tag.
      References
      Product Information
      PresentationSupplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.
      Key Applications
      • IP-Kinase Assay
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      Protein TargetAkt1/PKBα
      Purification MethodNi2+/NTA and glutathione agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight59.9kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size15 µg
      Material PackageAlso available in 250μg size, please inquire for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      14-276 04053252590139

      Documentation

      Akt1/PKBα Protein, active, 15 µg SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Akt1/PKBα Protein, active, 15 µg Analysenzertifikate

      TitelChargennummer
      Akt1/PKB#225;, active - D8MN034U-M D8MN034U-M
      Akt1/PKB#945;, active - D9BN042U-E D9BN042U-E
      Akt1/PKB#945;, active - D9BN042U-F D9BN042U-F
      Akt1/PKB#945;, active - D9BN042U-G D9BN042U-G
      Akt1/PKB#945;, active - D9BN042U-H D9BN042U-H
      Akt1/PKB#945;, active - D9BN042U-I D9BN042U-I
      Akt1/PKB#945;, active - D9BN042U-J D9BN042U-J
      Akt1/PKB#945;, active - D9BN042U-K D9BN042U-K
      Akt1/PKB#945;, active - D9BN042U-L D9BN042U-L
      Akt1/PKBa, active - D9BN042U_R D9BN042U…R

      Literatur

      ÜbersichtAnwendungPub Med ID
      Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
      Mîinea, Cristinel P and Lienhard, Gustav E
      BioTechniques, 34: 828-31 (2003)  2003

      Kinase Assay12703308 12703308
      Heat shock protein 27 controls apoptosis by regulating Akt activation.
      Rane, Madhavi J, et al.
      J. Biol. Chem., 278: 27828-35 (2003)  2003

      Abstract anzeigen
      Kinase Assay12740362 12740362
      Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells
      Bauer, B., et al
      J Biol Chem, 276:31627-34 (2001)  2001

      Kinase Assay11410591 11410591
      A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
      Frame, S., et al
      Mol Cell, 7:1321-7 (2001)  2001

      Kinase Assay11430833 11430833
      3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates and activates the p70 S6 kinase in vivo and in vitro.
      Alessi, D R, et al.
      Curr. Biol., 8: 69-81 (1998)  1998

      9427642 9427642
      Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha.
      Alessi, D R, et al.
      Curr. Biol., 7: 261-9 (1997)  1997

      9094314 9094314
      PDK1, one of the missing links in insulin signal transduction?
      Cohen, P, et al.
      FEBS Lett., 410: 3-10 (1997)  1997

      Abstract anzeigen
      9247112 9247112

      Verwandte Produkte & Anwendungen

      Alternative Packsize

      Bestellnummer Beschreibung  
      14-276M Akt1/PKBα Protein, active, 250 µg Preis & Verfügbarkeit

      Produktfamilien

      Kategorien

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases