Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

70186 pT7Blue-2 Blunt Vector (linearized) - Novagen

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70186
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.





The pT7Blue-2 vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), f1 origin of replication and modified multiple cloning region designed for optimal expression of cloned inserts by in vitro translation. The multiple cloning region contains a T7 promoter upstream of the Xenopus β-globin 5′ UTR translation enhancer and S•Tag™ sequences, followed by an enterokinase cleavage site and extensive region of cloning sites. An SP6 promoter is placed within the multiple cloning sites to allow the synthesis of essentially unfused RNA from DNA inserted downstream.

Catalogue Number70186
Brand Family Novagen®
References
References

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
Product Information
Components
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
70186 0

Documentation

pT7Blue-2 Blunt Vector (linearized) - Novagen Certificates of Analysis

TitleLot Number
70186

References

Přehled odkazů

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB183 Perfectly Blunt® Cloning Kits

Vector Sequence

Title
pT7Blue-2 Sequence

Související produkty a aplikace

Related Products

Katalogové číslo Popis
70075 Introductory pT7Blue-3 Perfectly Blunt® Cloning Kit
70174 pT7Blue Blunt Vector (linearized) - Novagen
70182 pT7Blue-3 Perfectly Blunt® Cloning Kit - Novagen
70183 Introductory pT7Blue Perfectly Blunt® Cloning Kit - Novagen
70184 Introductory pSTBlue-1 Perfectly Blunt® Cloning Kit - Novagen
70188 pSTBlue-1 Blunt Vector (linearized) - Novagen
70189 pT7Blue Perfectly Blunt® Cloning Kit - Novagen
70191 pSTBlue-1 Perfectly Blunt® Cloning Kit - Novagen
70633 Introductory pETBlue-1 Perfectly Blunt® Cloning Kit - Novagen
70634 pETBlue-1 Perfectly Blunt® Cloning Kit - Novagen
Show more

Související produkty: Application Facete

PCR and Cloning

Kategorie

Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors