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71339 pIEx™-7 Ek/LIC Vector Kit - Novagen

Novagen's pIEx-7 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus.

pIEx™-7 Ek/LIC Vector Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: InsectDirect™ pIEx™-7 Ek/LIC Vector Kit

71339
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Přehled

Replacement Information

Products

Katalogové čísloBalení ks/bal.
71339-3 Plastová ampulka 20 rxn
Description
OverviewThe pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1).
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number71339
Brand Family Novagen®
SynonymsInsectDirect™ pIEx™-7 Ek/LIC Vector Kit
References
ReferencesKim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
Product Information
•1 µgEk/LIC Vector
•25 unitsT4 DNA Polymerase, LIC-qualified
•50 µl25 mM EDTA
•40 µl25 mM dATP
•8 µlEk/LIC β-gal Control Insert
•100 µl100 mM DTT
•50 µl10X T4 DNA Polymerase Buffer
•1.5 mlNuclease-free Water
•5 × 2 mlSOC Medium
•10 µlTest Plasmid
•22 × 50 µlNovaBlue GigaSingles™ Competent Cells
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
71339-3 04055977253269

Documentation

pIEx™-7 Ek/LIC Vector Kit - Novagen Certificates of Analysis

TitleLot Number
71339

References

Přehled odkazů
Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

Brochure

Title
Insect Cell Expression
Insect Directﾙ Protein Expression & Purification System
Radiance! Multisystem Protein Expression

Citations

Název
  • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
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    User Protocols

    Title
    TB163 Ek/LIC Cloning Kits

    Vector Map

    Title
    TB399VM pIEx-7 Ek/LIC Vector Map

    Vector Sequence

    Title
    pIEx-7 Ek/LIC Vector Sequence