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70775 pET Expression System 27b

pET vectors plus host strains with induction control

pET Expression System 27b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 27b
The pET-27b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB073VM.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number70775
Brand Family Novagen®
References
Product Information
•10 µgpET-27b(+) vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•0.2 mlInduction control clone, glycerol stock
Fusion tagHis•Tag, HSV•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
70775 0

Documentation

pET Expression System 27b Certificates of Analysis

TitleLot Number
70775

Citations

Název
  • Jason Cellitti, et al. (2007) Exploring subdomain cooperativity in T4 lysozyme I: Structural and energetic studies of a circular permutant and protein fragment. Protein Science 16, 842-851.
  • Asa Flanigan and Jeffrey F. Gardner. (2007) Interaction of the gifsy-1 xis protein with the gifsy-1 attP sequence. Journal of Bacteriology 189, 6303-6311.
  • R. Jeremy Johnson, Shawn R. Lin and Ronald T. Raines. (2007) Genetic selection reveals the role of a buried, conserved polar residue. Protein Science 16, 1609-1616.
  • Sebastian Kalamajski, Anders Aspberg and Ake Oldberg. (2007) The decorin sequence SYIRIADTNIT binds collagen type I. Journal of Biological Chemistry 282, 16062-16067.
  • Danxu Liu, Paul Crellin and Ronald Chalmers. (2005) Cyclic changes in the affinity of protein-DNA interactions drive the progression and regulate the outcome of the Tn10 transposition reaction. Nucleic Acids Research 33, 1982-1992.
  • Christian Schwegler, et al. (2005) Monoclonal anti-idiotype antibody 6G6.C4 fused to GM-CSF is capable of breaking tolerance to carcinoembryonic antigen (CEA) in CEA-transgenic mice. Cancer Research 65, 1925-1933.
  • Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry 276, 23992-23999.
  • Jeffrey J. Brandt, Lisa L. Chatwood and Michael W. Crowder. (2000) Analysis of three overexpression systems for VanX, the zinc(II) dipeptidase required for high-level vancomycin resistance in bacteria. Protein Expression and Purification 20, 300-307.
  • B. Kenny, et al. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into ammmalian cells. Cell 91, 511-520.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB073VM pET-27b(+) Vector Map

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    Kategorie

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors