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ASK07 p53 Antibody Sampler Kit

p53 Antibody Sampler Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ASK07
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species Reactivity
A Broad Range Of Species
Description
Overview

This product has been discontinued.



20 µg of various p53 antibodies. Includes Cat. Nos. OP03, OP09, OP29, OP32, OP33, and OP43.
Please see individual product descriptions for more information on applications and species reactivity.
Catalogue NumberASK07
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Product Information
FormLiquid
Formulation20 µg of each antibody in 200 µl (100 µg/ml) of 0.05 M sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application NotesImmunoblotting (see characteristics below)
Paraffin Sections (see characteristics below)
Frozen Sections (see characteristics below)
Immunoprecipitation (see characteristics below)
Immunofluorescence (see characteristics below)
Application CommentsCat. No. OP03, Cat. No. OP09 and Cat. No. OP43 are pantropic (detect both mutant and wild-type p53). Cat. No. OP32 and Cat. No. OP33 detect only wild-type p53. Cat. No. OP29 detects only mutant p53 under nondenaturing conditions (immunoprecipitation, immunofluorescence and frozen sections) but is pantropic under denaturing conditions (immunoblotting and paraffin sections). Wild-type p53 has a short half-life and is present in low amounts; thus, it can be difficult to detect. For immunoprecipitations, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. To increase sensitivity in a western blot, load more sample (i.e. 150 mg total cell protein per lane) and/or increase the concentration of primary antibody. To minimize extra bands in a western blot, reduce the concentration of both primary and secondary antibodies. Breast carcinoma can be used as a positive control (40% will be positive) for frozen and paraffin sections. p53 (Ab-3) will give light staining of paraffin sections after pretreating with 6M urea for 10 min at 95°C. Ab-4 and Ab-5 have been used in frozen sections but are not recommended for immunoblotting. All antibodies in this kit are too dilute to be used in gel shift assays.
Biological Information
Species Reactivity
  • A Broad Range Of Species
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ASK07 0

Documentation

p53 Antibody Sampler Kit Certificates of Analysis

TitleLot Number
ASK07

References

Přehled odkazů
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
p53 And Related Products
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
ApplicationImmunoblotting (see characteristics below)
Paraffin Sections (see characteristics below)
Frozen Sections (see characteristics below)
Immunoprecipitation (see characteristics below)
Immunofluorescence (see characteristics below)
BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
Speciesa broad range of species
FormLiquid
Formulation20 µg of each antibody in 200 µl (100 µg/ml) of 0.05 M sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
Preservative≤0.1% sodium azide
CommentsCat. No. OP03, Cat. No. OP09 and Cat. No. OP43 are pantropic (detect both mutant and wild-type p53). Cat. No. OP32 and Cat. No. OP33 detect only wild-type p53. Cat. No. OP29 detects only mutant p53 under nondenaturing conditions (immunoprecipitation, immunofluorescence and frozen sections) but is pantropic under denaturing conditions (immunoblotting and paraffin sections). Wild-type p53 has a short half-life and is present in low amounts; thus, it can be difficult to detect. For immunoprecipitations, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. To increase sensitivity in a western blot, load more sample (i.e. 150 mg total cell protein per lane) and/or increase the concentration of primary antibody. To minimize extra bands in a western blot, reduce the concentration of both primary and secondary antibodies. Breast carcinoma can be used as a positive control (40% will be positive) for frozen and paraffin sections. p53 (Ab-3) will give light staining of paraffin sections after pretreating with 6M urea for 10 min at 95°C. Ab-4 and Ab-5 have been used in frozen sections but are not recommended for immunoblotting. All antibodies in this kit are too dilute to be used in gel shift assays.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.