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71552 iFOLD® Protein Refolding System 1

Purify inclusion bodies and determine optimal protein refolding conditions in a 96-well plate

iFOLD® Protein Refolding System 1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Structural and functional proteomics requires large amounts of very pure and correctly folded protein. Due to simplicity, low cost, and potential to express large quantities of target protein, E. coli expression systems are frequently used. However, production of foreign proteins in E. coli may result in the formation of inclusion bodies. Although inclusion body formation is typically not desirable, the aggregates are resistant to proteolysis, easily purified, and can be denatured with chaotropes. Unfortunately, defining conditions that promote refolding of the target protein into its native conformation is empirical and difficult.

The iFOLD® Protein Refolding System 1 is designed to determine optimal protein refolding conditions by a systematic evaluation of 92 different buffers covering a range of pH and different salt, cyclodextrin, redox agent, and refolding additive concentrations. The iFOLD system matrix design is based on an extensive literature review of successful refolding experiments and on information contained in the REFOLD database (http://refold.med.monash.edu. au). The system includes inclusion body purification reagents combined with a 96-well plate-based protein refolding matrix. Following cell lysis, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of detergent and buffer washes. The purified inclusion bodies are denatured by addition of TCEP [Tris(2-carboxyethyl)phosphine] and N-Lauroylsarcosine and refolded by rapid dilution into the iFOLD 96-well buffer matrix. Each kit is sufficient for screening up to 96 refolding conditions (92 experimental and four control wells) for a single protein.

Description
Catalogue Number71552 Brand Family Novagen®
Features and benefits• All reagents for inclusion body purification and pre-dispensed iFOLD™ 96-well protein refolding buffer matrix
• Comprehensive set of optimized screening conditions for protein refolding
• Refolding conditions based on extensive literature review
• 96-well refolding plate compatible with HT methodology
References
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Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple storage requirements
Do not freeze Ok to freeze
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Katalogové číslo GTIN
71552 0