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QIA128 Ultrasensitive Cell Proliferation Assay Kit, Fluorogenic

QIA128
  
Purchase on Sigma-Aldrich

Přehled

Tabulka spec. kláve

Detection Methods
Fluorometric
Description
Overview

This product has been discontinued.





A highly sensitive, reliable cell proliferation assay utilizing Calcein-AM, a fluorescent probe for staining viable cells (Ex.: ~485 nm; Em.: ~520 nm). For optimal results serum-free media or Dulbecco’s PBS is recommended. Endogenous esterases in serum can lead to increased blank values which can be subtracted out. Extremely useful for cytotoxicity assays.
Catalogue NumberQIA128
Brand Family Calbiochem®
Materials Required but Not Delivered Pipettors with disposable tips
Tissue culture grade, flat-bottom 96-well black plate
Fluorescence reader capable of measuring fluorescence in 96-well plates with a filter set of ~485/520 nm
References
ReferencesYang, A., et al. 2002. Cell Biol. Toxicol. 18, 97.
De Clerk, L.S. 1994. J. Immunol. Methods 172, 115.
Bozyczko-Coyne, D., et al. 1993. J. Neurosci. Methods 50, 205
Wang, X.M., et al. 1993. Hum. Immunol. 37, 264.
Product Information
Detection methodFluorometric
Form500 Tests
Format96-well plate
Kit containsCalcein-AM, Dulbecco-PBS.
Biological Information
Assay range250-25,000 cells
Assay time1 h
Sample TypeViable cells
Physicochemical Information
Sensitivity100 Cells
Product Usage Statements
Intended useThe Calbiochem® Ultrasensitive Cell Proliferation Assay Kit is designed to fluorometrically determine the number of viable cells in proliferation, or for cytotoxicity assays that require greater sensitivity than absorbance-based proliferation assays. The amount of fluorescent dye produced by cellular esterases is proportional to the number of viable cells. The detection range for the number of viable cells is from 100 to 30,000 cells. Due to this high sensitivity the Calbiochem Ultrasensitive Cell Proliferation Assay Kit is also suitable for staining cells in cell-based studies such as cell adhesion or chemotaxis assays.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
Protect from Light Protect from light
Do not freeze Ok to freeze
Supplemental Information
Kit containsCalcein-AM, Dulbecco-PBS.
Global Trade Item Number
Katalogové číslo GTIN
QIA128 0