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Stericup® Filter Units

The Stericup vacuum filtration system combines a Steritop vacuum filtration unit with a receiver flask for processing and storing volumes from 150 mL to 1,000 mL.

The Stericup vacuum filtration system combines a Steritop filter unit with a receiver flask for processing and storing volumes from 150 mL to 1,000 mL.

Its new no tip/easy grip flask design and compact profile improve stability during filtration, make gripping the receiver easier, and make Stericup Filter Units ideal for use in laminar flow hoods.

As an added convenience, the bottom of the receiver flask is slightly recessed, enabling capped flasks to be stacked for convenient storage.

Overview

Specifications

Ordering Information

Jít
Units with fast flow and low protein binding 0.1 µm Millipore Express (PES) membrane for sterilization of aqueous solutions and mycoplasma removal; supplied sterileVymazat řazení a filtrování Show Filter
Katalogové čísloDescriptionFunnel CapacityReceiverPack Size
SCVPU02REStericup-VP Sterile Vacuum Filtration System 250 mL 250 mL bottle with cap 12 pack, VP, 0.10 µm, polyethersulfone, 250 mL, radio-sterilized

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Units with fast flow and low protein binding 0.22 µm Millipore Express PLUS (PES) membrane for sterilization of aqueous solutions; supplied sterile*Vymazat řazení a filtrování Show Filter
Katalogové čísloDescriptionFunnel CapacityReceiverPack Size
SCGPU01REStericup-GP Sterile Vacuum Filtration System 150 mL 150 mL bottle with cap 12 pack, GP, 0.22 µm, polyethersulfone, 150 mL, radio-sterilized

* These products have been tested for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Stericup-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Stericup-GP filtration does not impact pluripotency of mouse stem cells.

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Units with very low protein binding 0.22 µm Durapore (PVDF) membrane for sterilization of aqueous solutions; supplied sterileVymazat řazení a filtrování Show Filter
Katalogové čísloDescriptionFunnel CapacityReceiverPack Size
SCGVU05REStericup-GV Sterile Vacuum Filtration System 500 mL 500 mL bottle with cap 12

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Documentation

FAQ

QuestionAnswer
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the typical holdup volume for Stericup.The hold up volume is about 6 ml. If you give the unit a slight shake, you can reduce the holdup volume by about 60% to 2.4 ml. Solutions containing protein have a slightly higher holdup volume, about 7 ml, again reduced to less than half by a slight shake.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
Should I filter my acrylamide solution prior to gel casting?Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.

Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
It sounds like air is leaking into my Stericup, what should I do?First make sure that the top is securely fastened to the base. Discontinue use if there appears to be any cracks on the apparatus.
What Stericup would you recommend to remove mycoplasma?The Stericup VP ontains an 0.1um Express membrane. The VP membrane has been validated for the removal of mycoplasma.
Which pre-filter should I be using with my Stericup or Steritop unit?You should use an AP20 75mm pre-filter (AP2007500). To help keep the filter from floating in the solution to be filtered, insert the pre-filter under the pre-filter tab inside the Stericup or Steritop.
What is the expiration date on Stericup and Steritop units?The expiration date, which specifies product sterility, for the Stericup and Steritop products is three years from the date of manufacture.
What type of flow rates can I expect with the Stericups and Steritops?For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.
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Stericup®

Kategorie

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices