Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

524744 SIRT2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sirtuin 2, His•Tag®, Human, Recombinant, E. coli

524744
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human SIRT2 (amino acids 50-356) fused at the C-terminus to a His•Tag® sequence and expressed in E. coli. SIRT2 is a member of the class III histone deacetylase family and its activity is dependent on the co-factor, NAD+, which is cleaved during the deacetylation reaction. Sirtuins also possess an ADP-ribosyltransferase activity, that is unique to the class III HDACs. The active deacetylase is useful in the study of SIRT2 regulation and inhibitor screening.
Catalogue Number524744
Brand Family Calbiochem®
SynonymsSirtuin 2, His•Tag®, Human, Recombinant, E. coli
References
ReferencesGrubisha, O., 2006. J.Biol.Chem. 281, 14057.
Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.
Product Information
Unit of DefinitionOne unit is defined as the amount of enyme that will deacetylate 1 picomol fluorescent peptide substrate, Arg-His-Lys-Lys(Ac), per min at 30°C.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 0.05% Tween®-20, 50% glycerol, pH 8.0.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥1.4 U/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
524744 0

Documentation

SIRT2, His•Tag®, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

SIRT2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
524744

References

Přehled odkazů
Grubisha, O., 2006. J.Biol.Chem. 281, 14057.
Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.

Data Sheet

Title
SIRTainty™ Class III HDAC Assay
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-April-08 JSW
SynonymsSirtuin 2, His•Tag®, Human, Recombinant, E. coli
DescriptionRecombinant, human SIRT2 (amino acids 50-356) fused at the C-terminus to a His•Tag® sequence and expressed in E. coli. SIRT2 is a member of the class III histone deacetylase family and its activity is dependent on the co-factor, NAD+, which is cleaved during the deacetylation reaction. Sirtuins also possess an ADP-ribosyltransferase activity, that is unique to the class III HDACs. The active deacetylase is useful in the study of SIRT2 regulation and inhibitor screening.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 0.05% Tween®-20, 50% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, 500 µM NAD+, 20 µM substrate [rg-His-Lys-Lys(Ac)], 100 ng/µl SIRT2, 37°C
Purity≥90% by SDS-PAGE
Specific activity≥1.4 U/µg protein
Unit definitionOne unit is defined as the amount of enyme that will deacetylate 1 picomol fluorescent peptide substrate, Arg-His-Lys-Lys(Ac), per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesGrubisha, O., 2006. J.Biol.Chem. 281, 14057.
Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.