Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-622M SHP-2 Protein, 250 µg

N-terminal GST-tagged recombinant, human SHP-2 amino acids 230-545, for use in Phosphatase Assays.

SHP-2 Protein, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-622M
250 µg  Also available in 10 μg size (cat#14-622) and in bulk (cat#14-622-K).
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue Number14-622M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSHP-2 Protein, 250 µg
OverviewN-terminal GST-tagged recombinant, human SHP-2 amino acids 230-545
References
Product Information
Quality LevelMQ300
Applications
ApplicationN-terminal GST-tagged recombinant, human SHP-2 amino acids 230-545, for use in Phosphatase Assays.
Key Applications
  • Phosphatase Assay
Biological Information
Sourcehuman SHP-2 expressed in E. coli
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Gene Symbol
  • PTPN11
  • PTP-2C
  • SH-PTP2
  • SHP-2
  • PTP2C
  • BPTP3
  • SHP2
  • SH-PTP3
  • MGC14433
  • Shp2
  • PTP-1D
  • SHPTP2
  • CFC
  • NS1
Protein TargetSHP-1
Purification MethodGlutathione-agarose
Target Sub-FamilyTyr phosphatase
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q06124 # Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
SIZE: 597 amino acids; 68436 Da
SUBUNIT: Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL6 and ANKHD1.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, with highest levels in heart, brain, and skeletal muscle.
DOMAIN: SwissProt: Q06124 The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
PTM: Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
DISEASE: SwissProt: Q06124 # Defects in PTPN11 are the cause of LEOPARD syndrome [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. & Defects in PTPN11 are the cause of Noonan syndrome 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant. & Defects in PTPN11 are a cause of Noonan-like syndrome [MIM:163955]; also known as Noonan-like/multiple giant cell lesion syndrome. It is an autosomal dominant disorder characterized by Noonan features associates with giant cell lesions of bone and soft tissue. & Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte- macrophage colony stimulating factor.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. & Contains 2 SH2 domains. & Contains 1 tyrosine-protein phosphatase domain.
Molecular Weight63.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by the dephosphorylation of 100μM DiFMUP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-622) and in bulk (cat#14-622-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
14-622M 04053252827020