Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

HTS036RTA Ready-to-Assay™ CCR9 Chemokine Receptor Frozen Cells

Human CCR9 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_031200
HTS036RTA
1 kit  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

This product requires a User Agreement (Label License). Please see product datasheet for further information

Tabulka spec. kláve

SpeciesHost CellsProtein Target
Human Chem-1CCR9
Description
Catalogue NumberHTS036RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ CCR9 Chemokine Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The CCR9 receptor is a GPCR expressed selectively in the thymus, lymph node, spleen, and the small bowel (SB). CCR9 binds to Thymus-expressed chemokine ligand (TECK / CCL25) (Zaballos et al. 1999, Yu et al., 2000, Carramolino et al., 2001), and is involved in directing the movement of mature T cells in the thymus. CCR9 also plays an important role in the pathogenesis of SB Crohn’s disease (Papadakis et al, 2001). The CCR9 selective expression in the small bowel but not the colon indicates the the role of CCR9 in the specialized immune response in the SB. It was also shown that CCR9-mediated antiapoptosis could help in the study of cell survival mechanism and T cell malfunctioning. Millipore’s cloned human CCR9-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR9 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at CCR9.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman CCR9 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetCCR9
Target Sub-FamilyChemokine
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Global Trade Item Number
Katalogové číslo GTIN
HTS036RTA 04053252681332