SCC007 Sigma-AldrichReNcell® CX Human Neural Progenitor Cell Line
ReNcell® CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.
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Catalogue Number | SCC007 |
Brand Family | Chemicon® |
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Description | ReNcell® CX Human Neural Progenitor Cell Line |
Overview | ReNcell® CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell® CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell® CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell® CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell® CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell® CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures. ReNcell Now Used in a 3D Cell Model of Alzheimer's Disease! Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer's disease. Nature. 2014 Nov 13;515(7526):274-8. (Published in Nature) |
Materials Required but Not Delivered | 1. ReNcell® NSC Maintenance Medium (Millipore Cat. No. SCM005) 2. ReNcell® NSC Freezing Medium (Millipore Cat. No. SCM007) 3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 106 Units/mg. Millipore Cat. No. GF003) 4. Epidermal growth factor (EGF; Specific Activity > 1 x 107 Units/mg; Millipore Cat. No. GF001) 5. Laminin (Millipore Cat. No. CC095) 6. DMEM/F12 w/o HEPES, w/ L-Glutamine (Millipore Cat. No. DF-042-B) 7. Accutase (Millipore Cat. No. SCR005) 8. Tissue culture-ware 9. Phosphate-Buffered Saline (1X PBS) (Millipore Cat. No. BSS-1005-B) 10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS) 11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS) 12. Primary and secondary antibodies 13.4'-6-Diamidino-2-phenylindole (DAPI) / PBS Solution 14. Anti-fading mounting solution (DABCO/PVA) 15. Hemacytometer 16. Microscope |
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Quality Level | MQ100 |
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Application | ReNcell® CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells. |
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Source | Human cortical brain tissue |
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Quality Assurance | Each lot of ReNcell® CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C. |
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Material Size | 1 ea |
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Katalogové číslo | GTIN |
SCC007 | 04053252358609 |