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17-439 RalB Activation Assay Kit (non-radioactive)

Non-radioactive Ral-B Activation Assay Kit can be used to precipitate Ral-B-GTP from cell lysates & detection by a Ral-B specific monoclonal antibody.

RalB Activation Assay Kit (non-radioactive) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: G-Protein Assay
17-439
20 assays  
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Tabulka spec. kláve

Species ReactivityKey Applications
H, M, RGPA
Description
Catalogue Number17-439
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRalB Activation Assay Kit (non-radioactive)
Alternate Names
  • RAS-like protein B
  • v-ral simian leukemia viral oncogene homolog B
  • GTP binding protein
Materials Required but Not DeliveredStimulated and Non-stimulated Cell Lysates
Pipettes, Micropipettes and Tips
Electrophoresis and Immunoblotting Equipment
Microcentrifuge
Microcentrifuge Tubes
Ral Activators
Tissue Culture Supplies
4C Tube Rocker
Glycerol
aprotinin
PMSF
Anti-Mouse Secondary Antibody and Detection Reagents
TRIS Buffered Saline (TBS), Ice Cold
Product Information
Components
  • anti-RalB, clone 25 (Cat.# 04-037)
  • Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
Quality LevelMQ100
Applications
ApplicationNon-radioactive Ral-B Activation Assay Kit can be used to precipitate Ral-B-GTP from cell lysates & detection by a Ral-B specific monoclonal antibody.
Key Applications
  • G-Protein Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Entrez Gene Number
Gene Symbol
  • RALBP1
  • RLIP1
  • RIP1
  • RalBP1
  • RIP
  • RLIP76
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15311 # Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
SIZE: 655 amino acids; 76063 Da
SUBUNIT: Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDC2 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
SIMILARITY: SwissProt: Q15311 ## Contains 1 Rho-GAP domain.
Product Usage Statements
Quality AssuranceRoutinely evaluated by non-radioactive Kinase Assay.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C from date of shipment
Packaging Information
Material Size20 assays
Global Trade Item Number
Katalogové číslo GTIN
17-439 04053252730849