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SCC462 RMS RET Melanoma-sorted Mouse Cell Line

Unlike most existing preclinical model systems, SCC462 retains the capacity for spontaneous metastasis to the brain, the lungs, and other organs in immunocompetent mice following orthotopical inoculation by subdermal injection. SCC462 has been demononstrated to recapitulate the multi-stage process involving the interactions of disseminated metastatic cells with the brain microenvironment, including instigation of astrogliosis, neuroinflammation, and vascular hyperpermeability.

SCC462
>1x10^6 viable cells/vial   
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Replacement Information
Description
Catalogue NumberSCC462
DescriptionRMS RET Melanoma-sorted Mouse Cell Line
OverviewThe parental cells for SCC462 were isolated from a spontaneously occurring skin tumor in RET transgenic mice. The Ret oncogene was originally created by an accidental in vivo recombination of two human genes, Ret and RFP (TRIM27). SCC462, the mouse Ret-melanoma sorted (RMS) cell line, was created by engineering the isolated Ret-melanoma cells to express the puromycin resistance gene and the fluorescent reporter gene mCherry by retroviral transduction followed by fluorescence selection.
Alternate Names
  • RMS cell line
  • RET melanoma cell line
  • RET melanoma-sorted cell line
Background InformationThe RET oncogene was originally created by an accidental in vivo recombination of two human genes, RET and RFP (the RET-finger protein, known today as TRIM27), during a transformation of NIH3T3 cells with human lymphoma DNA (Takahashi 1985). The founder RET transgenic mouse strains were generated by the embryonic microinjection of the RFP-RET oncogene under the mouse metallothionein-I promoter-enhancer (Iwamoto 1991), and a spontaneous metastatic melanoma model line was established by repetitive rounds of back-crossing of one of such founders (Kato 1998).

SOURCE

The Ret-melanoma sorted cells (RMS) were derived from spontaneous metastatic skin tumors in Ret-transgenic mice. The founder transgenic mice were created by microinjection of the Ret transgene into the fertilized egg of (C57BL/6 x BALB/c) x BALB/c mice. A founder mouse was crossed with BCF1 (BALB/c x C57BL/6) mice to establish a transgenic line, designated “the 304 line.” When these F1 transgenic mice were subsequently crossed with C57BL/6 mice (coat color: black), BCF1 mice (agouti), and BALB/c mice (albino), benign melanocytic tumors often developed spontaneously in the pigmented F2 offspring, but did not progress to metastatic tumors. The spontaneous metastatic melanoma model (designated the 304/B6 line), in which the parent of the SCC462 cells arose, was generated by 10 rounds of back-crossing of the 304 line with C57BL/6 mice

REFERENCES

1. Takahashi M, Ritz J, Cooper GM. 1985. Activation of a novel human transforming gene, ret, by DNA rearrangement. Cell. 42(2): 581-588.
2. Iwamoto T, Takahashi M, Ito M, Hamatani K, Ohbayashi M, Wajjwalku W, Isobe K, Nakashima I. 1991. Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene. EMBO J. 10(11): 3167-3175.
3. Kato M, Takahashi M, Akhand AA, Liu W, Dai Y, Shimizu S, Iwamoto T, Suzuki H, Nakashima I. 1998. Transgenic mouse model for skin malignant melanoma. Oncogene. 17(14): 1885-1888.
4. Schwartz H, Blacher E, Amer M, Livneh N, Abramovitz L, Klein A, Ben-Shushan D, Soffer S, Blazquez R, Barrantes-Freer A, et al. 2016. Incipient melanoma brain metastases instigate astrogliosis and neuroinflammation. Cancer Res. 76(15):4359-4371.
5. Doron H, Amer M, Ershaid N, Blazquez R, Shani O, Lahav TG, Cohen N, Adler O, Hakim Z, Pozzi S, et al. 2019. Inflammatory activation of astrocytes facilitates melanoma brain tropism via the CXCL10-CXCR3 signaling axis. Cell Rep. 28(7): 1785-1798.
References
Product Information
Components
  • ≥1x106 viable RMS cells: (Catalog No. SCC462). Store in liquid nitrogen.
Quality LevelMQ100
Applications
ApplicationUnlike most existing preclinical model systems, SCC462 retains the capacity for spontaneous metastasis to the brain, the lungs, and other organs in immunocompetent mice following orthotopical inoculation by subdermal injection. SCC462 has been demononstrated to recapitulate the multi-stage process involving the interactions of disseminated metastatic cells with the brain microenvironment, including instigation of astrogliosis, neuroinflammation, and vascular hyperpermeability.
Key Applications
  • Cell Culture
  • In vitro Bioassay
  • Cell Based Assays
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
HostMouse
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥1X10⁶ viable cells.
• sBT-RMS cells are verified to be of mouse origin and negative for human, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells tested negative for infectious diseases against a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage ConditionsRMS cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size>1x10^6 viable cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
SCC462 04065270302144

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