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540222 Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem

Puromycin, Dihydrochloride, CAS 58-58-2, is An aminonucleoside antibiotic that inhibits protein synthesis by blocking the translation step and causes premature release of nascent polypeptide chains.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl

Primary Target: protein synthesis
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Přehled

Replacement Information

Tabulka spec. kláve

CAS #Empirical Formula
58-58-2C₂₂H₂₉N₇O₅ · 2HCl
Description
OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
Catalogue Number540222
Brand Family Calbiochem®
Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
References
ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
Wirth, M., et al. 1994. J. Virol. 68, 566.
Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.
Product Information
CAS number58-58-2
FormWhite to off-white solid
Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetprotein synthesis
Purity≥98% by TLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSAU7355000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
540222 0

Documentation

Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem Certificates of Analysis

TitleLot Number
540222

References

Přehled odkazů
Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
Wirth, M., et al. 1994. J. Virol. 68, 566.
Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.

Brochure

Title
Antibiotics Profiler
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-May-2008 RFH
Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
DescriptionProkaryotic and eukaryotic protein synthesis inhibitor. Nucleoside that resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Used for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product, puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Like cycloheximide, puromycin is also used as a general protein synthesis inhibitor in numerous cellular assays.
FormWhite to off-white solid
CAS number58-58-2
RTECSAU7355000
Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
Structure formulaStructure formula
Purity≥98% by TLC
SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 7943
ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
Wirth, M., et al. 1994. J. Virol. 68, 566.
Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.