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525188 Phospholipase C-γ-1, His•Tag®, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Phospholipase C-γ-1, Human, Recombinant

525188
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





Full-length, recombinant, human phospholipase C γ-1 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. PLC-γ-1 is a major substrate for heparin-binding growth factor 1-activated tyrosine kinase. Receptor-mediated activation of PLC-γ-1 and PLC-γ-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. The full-length protein contains the following domains: C2 domain (aa 1075-1177); EF-hand calcium-binding domain (aa 165-176); two PH domains (aa 27-142, aa 4889-523 and aa 895-931); PI-PLC X-box catalytic domain (aa 320-464); PI-PLC Y-box catalytic domain (aa 953-1070); two SH2 domains (aa 550-657, aa 668-756); and a SH3 domain (aa 791-851). Phosphorylation is known to occur at Tyr771 and Tyr1253 by EGFR, Tyr783 by Syk, Ser1248 by PKC-α, and Tyr432 and Tyr775 probably by FGFR.
Catalogue Number525188
Brand Family Calbiochem®
SynonymsPhospholipase C-γ-1, Human, Recombinant
Application Data
References
ReferencesHinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
Lennon, G., et al. 1996. Genomics 33, 151.
Product Information
FormLiquid
FormulationIn PBS.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
525188 0

Documentation

Phospholipase C-γ-1, His•Tag®, Human, Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

Phospholipase C-γ-1, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
525188

References

Přehled odkazů
Hinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
Lennon, G., et al. 1996. Genomics 33, 151.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-August-2008 RFH
SynonymsPhospholipase C-γ-1, Human, Recombinant
Application Data
DescriptionFull-length, recombinant, human phospholipase C γ-1 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. PLC-γ-1 is a major substrate for heparin-binding growth factor 1-activated tyrosine kinase. Receptor-mediated activation of PLC-γg-1 and PLC-γ-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. The full-length protein contains the following domains: C2 domain (aa 1075-1177); EF-hand calcium-binding domain (aa 165-176); two PH domains (aa 27-142, aa 4889-523 and aa 895-931); PI-PLC X-box catalytic domain (aa 320-464); PI-PLC Y-box catalytic domain (aa 953-1070); two SH2 domains (aa 550-657, aa 668-756); and a SH3 domain (aa 791-851). Phosphorylation is known to occur at Tyr771 and Tyr1253 by EGFR, Tyr783 by Syk, Ser1248 by PKC-α, and Tyr432 and Tyr775 probably by FGFR.
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Irritant
ReferencesHinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
Lennon, G., et al. 1996. Genomics 33, 151.