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ST1069 PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb

This PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of Paxillin (pSer¹⁷⁸).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1069
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody
Description
OverviewRecognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
Catalogue NumberST1069
Brand Family Calbiochem®
Application Data
Detection of mouse paxilin phosphorylated at Ser178, by immunoblotting. Samples: Whole cell lysate (~2.4 x 105 cells/lane) from NH3T3 cells serum-starved for 16 h and grown in suspension (lane 1) NIH3T3 cells plated on fibronectin (50 µg/ml for 1 h, lane 2), and NIH3T3 cells plated on fibronectin and treated with lamda phosphotase (lane 3). Primary antibody: Phospho-Dtect™ Anti-Paxilin (pSer178) Rabbit pAb (Cat. No. ST1069) (1:1000). Detection: chemiluminescence.
References
ReferencesBellis, S.L., et al. 1997. Biochem. J. 325, 375.
Schaller, M.D. 2001. Oncogene 20, 6459.
Huang, C., et al. 2003. Nature 424, 219.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate, pH 7.0-8.0.
Positive controlNIH3T3 cells plated on fibronectin
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000-1:5000)
Application CommentsDoes not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1069 0

Documentation

PhosphoDetect™ Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb Certificates of Analysis

TitleLot Number
ST1069

References

Přehled odkazů
Bellis, S.L., et al. 1997. Biochem. J. 325, 375.
Schaller, M.D. 2001. Oncogene 20, 6459.
Huang, C., et al. 2003. Nature 424, 219.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-October-2007 RFH
ApplicationImmunoblotting (1:1000-1:5000)
Application Data
Detection of mouse paxilin phosphorylated at Ser178, by immunoblotting. Samples: Whole cell lysate (~2.4 x 105 cells/lane) from NH3T3 cells serum-starved for 16 h and grown in suspension (lane 1) NIH3T3 cells plated on fibronectin (50 µg/ml for 1 h, lane 2), and NIH3T3 cells plated on fibronectin and treated with lamda phosphotase (lane 3). Primary antibody: Phospho-Dtect™ Anti-Paxilin (pSer178) Rabbit pAb (Cat. No. ST1069) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
BackgroundPaxillin is a focal adhesion adaptor protein associated with th establishment of focal adhesions, cell signaling via integrins, and cell motility. The N-terminus of paxillin contains a proline-rich region for SH3 and SH2 domain binding. The N-terminus also contains five copies of the LD motif for association of actin binding proteins. The C-terminus contains LIM domains and the focal adhesion targeting sequence. Paxillin is believed to function as an adaptor protein that facilitates assembly of multi-protein complexes and coordinates signaling via integrins. Tyrosine and serine phosphorylation of paxillin is associated with the establishment of cytoskeletal membrane attachments. Phosphorylation by JNK at Ser178 is necessary for normal cell motility.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin
IsotypeIgG
Specieshuman, mouse
Positive controlNIH3T3 cells plated on fibronectin
FormLiquid
FormulationIn Tris-citrate/phosphate, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.
Toxicity Highly Toxic
ReferencesBellis, S.L., et al. 1997. Biochem. J. 325, 375.
Schaller, M.D. 2001. Oncogene 20, 6459.
Huang, C., et al. 2003. Nature 424, 219.