Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DR1017 PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb

This PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of H2AX (pSer¹³⁹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DR1017
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form phosphorylated at Ser139.
Catalogue NumberDR1017
Brand Family Calbiochem®
Application Data
Detection of human and mouse γ-H2AX, phosphorylated at Ser139 by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer139) Rabbit pAb (1:10,000). Detection: chemiluminescence.
References
ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
Product Information
FormLiquid
FormulationIn Tris/citrate-phosphate buffer, pH 7.0-8.0.
Positive controlHEK293 cell nuclear extract
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:5000-1:15,000)
Immunocytochemistry (1:400-1:800)
Application CommentsDoes not recognize the unphosphorylated form. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹³⁹ phosphorylation site of human H2AX
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
DR1017 0

Documentation

PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb Certificates of Analysis

TitleLot Number
DR1017

References

Přehled odkazů
Stiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunoblotting (1:5000-1:15,000)
Immunocytochemistry (1:400-1:800)
Application Data
Detection of human and mouse γ-H2AX, phosphorylated at Ser139 by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer139) Rabbit pAb (1:10,000). Detection: chemiluminescence.
DescriptionImmunoffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquitinated form phosphorylated at Ser139.
BackgroundThe nucleosome is composed of ~200 bp of DNA and eight histone proteins, two from each of the four histone protein families, H4, H3, H2B, and H2A. H2A includes three subfamilies: H2A1-H2A2, H2AZ, and H2AX, which have been conserved throughout eukaryotic evolution. Phosphorylation of H2AX on serine 139 by ATM or DNA-dependent protein kinase (DNA-PK) is an early step in the response to DNA damage by ionizing radiation. Phosphorylation of H2AX is required for the accumulation of 53BP1 at DNA break sites.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹³⁹ phosphorylation site of human H2AX
IsotypeIgG
Specieshuman, mouse
Positive controlHEK293 cell nuclear extract
FormLiquid
FormulationIn Tris/citrate-phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not recognize the unphosphorylated form. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.