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539578 PKG, Iα, Bovine Lung

PKG, Iα, Bovine Lung MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα

539578
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Přehled

Replacement Information

Products

Katalogové čísloBalení ks/bal.
539578-4UG Plastová ampulka 4 μg
Description
OverviewNative PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
Note: 4µg = 6000 units.
Catalogue Number539578
Brand Family Calbiochem®
SynonymscGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
Application Data
References
ReferencesBrophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
FormLiquid
FormulationIn 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥1300 units/µg enzyme
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
539578-4UG 04055977194746

Documentation

PKG, Iα, Bovine Lung MSDS

Title

Safety Data Sheet (SDS) 

PKG, Iα, Bovine Lung Certificates of Analysis

TitleLot Number
539578

References

Přehled odkazů
Brophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
SynonymscGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
Application Data
DescriptionNative PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
FormLiquid
FormulationIn 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
Concentration Label Please refer to vial label for lot-specific concentration
Specific activity≥1300 units/µg enzyme
Unit definitionOne unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesBrophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.