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539494 PKC, Rat Brain

PKC, Rat Brain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase C

539494
  
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Přehled

Replacement Information
Description
OverviewNative PKC from rat brain predominantly containing the conventional PKC isoforms, α, β1, β2, and γ. A ubiquitous phospholipid-dependent enzyme involved in signal transduction associated with cell proliferation and differentiation. At least 10 PKC isoforms are reported to exist, which are classified as conventional (α, β, γ), novel (δ, e, η, θ, µ), and atypical (ξ, λ) isoforms.
M.W. 76,000-82,000.
Catalogue Number539494
Brand Family Calbiochem®
SynonymsProtein Kinase C
References
ReferencesChang, E.Y., et al. 1997. J. Immunol. 159, 2624.
Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
Nishizuka, Y. 1995. FASEB J. 9, 484.
Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.
Product Information
CAS number141436-78-4
Activity42 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to histone III-S per min at 22°C, pH 7.4.
EC number2.7.1.37
FormLiquid
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 1 mM DTT, 500 µM EDTA, 500 µM EGTA, 10% glycerol, pH 7.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥1000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Stable for up to 4 days at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
539494 0

Documentation

PKC, Rat Brain MSDS

Title

Safety Data Sheet (SDS) 

PKC, Rat Brain Certificates of Analysis

TitleLot Number
539494

References

Přehled odkazů
Chang, E.Y., et al. 1997. J. Immunol. 159, 2624.
Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
Nishizuka, Y. 1995. FASEB J. 9, 484.
Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-June-2009 RFH
SynonymsProtein Kinase C
DescriptionNative PKC from rat brain predominantly containing the conventional PKC isoforms, α, β1, β2, and γ. A ubiquitous phospholipid-dependent enzyme involved in signal transduction associated with cell proliferation and differentiation. At least 10 PKC isoforms are reported to exist, which are classified as conventional (α, β, γ), novel (δ, e, η, θ, µ), and atypical (ξ, λ) isoforms.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 1 mM DTT, 500 µM EDTA, 500 µM EGTA, 10% glycerol, pH 7.5.
CAS number141436-78-4
EC number2.7.1.37
Purity≥95% by SDS-PAGE
Specific activity≥1000 units/mg protein
Activity42 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to histone III-S per min at 22°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Stable for up to 4 days at 4°C.
Toxicity Standard Handling
ReferencesChang, E.Y., et al. 1997. J. Immunol. 159, 2624.
Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
Nishizuka, Y. 1995. FASEB J. 9, 484.
Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.