C153170Excellent support for synthesis of long and difficult peptides. Work synergistically with pseudoproline dipeptides.
NovaPEG resins are identical to Chemmatrix resins and are excellent solid supports for solid phase peptide and organic synthesis 1. Unlike other PEG-based polymer supports such as NovaSyn® TG and PEGA resins which contain either polystyrene or polyacrylamide backbones, NovaPEG resin contains only PEG units. This unique composition confers excellent swelling and mechanical properties on the polymer. The resin beads have swelling properties similar to PEGA resins, but unlike PEGA resins are free flowing beads in the dry state which makes them much easier to handle. Furthermore, in contrast to polystyrene and other commonly used supports, NovaPEG resin appears not to suffer from osmotic shock when solvent is exchanged from hydrophobic to hydrophilic solvents. NovaPEG resin also has excellent chemical stability, particularly towards strong acids and bases.
The amphiphilic nature of this resin makes it an excellent support for the synthesis of difficult, aggregated peptides and of long peptides and small proteins. In a reported synthesis of Bacuma, a 38-residue potential synthetic vaccine, the use of polystyrene-based resin gave an extremely heterogeneous product whereas NovaPEG Rink Amide resin afforded the target in excellent purity and yield 1. More remarkable is the result obtained from the synthesis of β-amyloid (1-42)1. This sequence is notoriously difficult to prepare owing to its propensity to aggregate. Using NovaPEG resin, this extremely problematic peptide was obtained in a crude purity of 91% using standard Fmoc SPPS methods. NovaPEG resin also gave a spectacular result in the synthesis of HIV-protease, affording an almost homogeneous product after 78 amino acid additions 2.
Remarkable synergies have been observed when they are used in combination with pseudoproline dipeptides. For example, the syntheses of the chemokines Rantes 3 and CCL4-L14 could only be achieved by employing both NovaPEG resins and pseudoproline dipeptides.
1. F. Garcia-Martin, et al. (2006) J. Comb. Chem., 8, 213.
2. S. Frutos, et al. (2007) Int. J. Pept. Res. Ther., 13, 221.
3. F. Garcia-Martin, et al. (2006) Biopolymers, 84, 566.
4. B. G. de la Torre, et al. (2007) Int. J. Pept. Res. Ther., 13, 265.
