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FCMAB140P Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18

Detect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BCL2, Bcl-2 UniProt Number: P10415
FCMAB140P
100 tests  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCRbPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB140P
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18
Alternate Names
  • B-cell CLL/lymphoma 2
    B-cell lymphoma protein 2
Background InformationThe Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.
References
Product Information
FormatPhycoerythrin
Control
  • Jurkat cells.
PresentationPurified rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Bcl-2 phosphorylated at Ser70.
EpitopeSer70
Clone69-10C-2-10C-18
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes phosphorylated Bcl-2 at Ser70.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
Gene Symbol
  • BCL2
  • Bcl-2
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).


SUBUNIT STRUCTURE: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.


SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.


TISSUE SPECIFICTY: Expressed in a variety of tissues.


DOMAIN: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.


PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) By similarity.


Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.


SEQUENCE SIMLARITIES: Belongs to the Bcl-2 family.
Molecular Weight26 kDa Observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
FCMAB140P 04053252411687

Documentation

Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18 MSDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18 Certificates of Analysis

TitleLot Number
Milli-MarkTM Anti-phospho-Bcl-2 (Ser70) -PE, clone 69-10C-2-10C-18 - 2021738 2021738
Milli-MarkTM Anti-phospho-Bcl-2 (Ser70) -PE, clone 69-10C-2-10C-18 - NRG1753942 NRG1753942
Milli-MarkTM Anti-phospho-Bcl-2 (Ser70) -PE, clone 69-10C-2-10C-18 -2512065 2512065
Milli-MarkTM Anti-phospho-Bcl-2 -2664438 2664438