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14-537M MKK6/SKK3 (S599D, T603D) Protein, active, 250 µg

Active, N-terminal MBP tagged, recombinant, human MKK6/SKK3 residues 4-end containing S599D & T603D mutations. For use in Kinase Assays.

14-537M
250 µg  Also available in 10 μg size (cat#14-537) and in bulk (cat#14-537-K).
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Replacement Information
Description
Catalogue Number14-537M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMKK6/SKK3 (S599D, T603D) Protein, active, 250 µg
OverviewN-terminal MBP tagged, recombinant, human MKK6/SKK3 residues 4-end containing S599D and T603D mutations.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal MBP tagged, recombinant, human MKK6/SKK3 residues 4-end containing S599D & T603D mutations. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman MKK6/SKK3 expressed in E. coli
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Gene Symbol
  • MAP2K6
  • PRKMK6
  • MEK6
  • SAPKK3
  • MAPKK6
  • MKK6
Protein TargetMKK6
Purification MethodAmylose Agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P52564 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
SIZE: 334 amino acids; 37492 Da
SUBUNIT: Interacts with Yersinia yopJ.
TISSUE SPECIFICITY: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.
PTM: Weakly autophosphorylated. & Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
SIMILARITY: SwissProt: P52564 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight80.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of p38α/SAPK2a, unactive (14-252).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-537) and in bulk (cat#14-537-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
14-537M 04055977159189

Documentation