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PRCYTMAG40K-PX25 MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
PRCYTMAG40K-PX25
96-well plate  
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Tabulka spec. kláve

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
sCD137 Eotaxin/CCL11 sFasL FGF-2 Fractalkine/CX3CL1 Granzyme A Granzyme B IL-1α IL-2 IL-4 IL-6 IL-16 IL-17A IL-17E/IL-25 IL-21 IL-22 IL-23 IL-28A IL-31 IL-33 IP-10/CXCL10 MIP-3α/CCL20 Perforin RANTES/CCL5 TNFβ/LTA NhPMplexLuminex xMAP
Description
Catalogue NumberPRCYTMAG40K-PX25
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel 2 enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background InformationCytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.
Product Information
Components
  • Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized
  • Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized
  • Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized
  • Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized
  • Sample Plate, 96 well format, 1 plate
  • 2 Foil plate sealers
  • Assay Buffer, 2 bottles, 30 mL
  • Bead Diluent, 1 bottle, 3.5 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
  • Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL
  • Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
  • Premixed 24-plex Beads, 1 bottle, 3.5 mL
  • RANTES beads, 1 vial
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Inter-assay: <15
  • Intra-assay: <10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of 1:2 diluted plasma or serum or 25 μL cell culture supernatant per well.
• RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Non-human Primate
Analytes Available
  • sCD137
  • Eotaxin/CCL11
  • sFasL
  • FGF-2
  • Fractalkine/CX3CL1
  • Granzyme A
  • Granzyme B
  • IL-1α
  • IL-2
  • IL-4
  • IL-6
  • IL-16
  • IL-17A
  • IL-17E/IL-25
  • IL-21
  • IL-22
  • IL-23
  • IL-28A
  • IL-31
  • IL-33
  • IP-10/CXCL10
  • MIP-3α/CCL20
  • Perforin
  • RANTES/CCL5
  • TNFβ/LTA
Cross ReactivityThis multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • RANTES/CCL5 (tissue culture): 1 – 750 pg/mL
  • IL-17A: 1 – 1,500 pg/mL
  • Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL
  • Granzyme B: 4 – 4,000 pg/mL
  • MIP-3α/CCL20: 7 – 7,000 pg/mL
  • sFasL: 8 – 8,000 pg/mL
  • sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL
  • IL-21, IL-31: 15 – 15,000 pg/mL
  • FGF-2, IL-33: 20 – 20,000 pg/mL
  • IL-23: 24 – 25,000 pg/mL
  • IL-1α, IL-28A: 29 – 30,000 pg/mL
  • IL-16: 73 – 75,000 pg/mL
  • Granzyme A: 78 – 80,000 pg/mL
  • Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL
  • IL-4: 146 – 150,000 pg/mL
  • IL-17E/IL-25: 293 – 300,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Katalogové číslo GTIN
PRCYTMAG40K-PX25 04055977133028