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444146 MDM2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mouse Double Minute 2, Human, Recombinant, E. coli

444146
  
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Replacement Information
Description
Overview

This product has been discontinued.

We apologize for the inconvenience, but we do not currently have an alternative product. For related products, please refer to our complete listing of proteasome and ubiquitination enzymes.





Recombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
Catalogue Number444146
Brand Family Calbiochem®
SynonymsMouse Double Minute 2, Human, Recombinant, E. coli
References
ReferencesBond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
Dornan, D., et al. 2004. Nature 429, 86.
Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.
Product Information
Unit of DefinitionOne unit is defined as 1 ng purified protein.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Applications
Biological Information
Biological activity20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay.
Purity> 95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
444146 0

Documentation

MDM2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
444146

References

Přehled odkazů
Bond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
Dornan, D., et al. 2004. Nature 429, 86.
Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-November-2007 JSW
SynonymsMouse Double Minute 2, Human, Recombinant, E. coli
DescriptionRecombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Purity> 95% by SDS-PAGE
Biological activity20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay.
Unit definitionOne unit is defined as 1 ng purified protein.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesBond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
Dornan, D., et al. 2004. Nature 429, 86.
Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.