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SCC635 Jurkat JE6.1 NF-kB::eGFP Cell Line

Jurkat JE6.1 NF-ĸB::eGFP cell line expresses only the endogenous TLR6 on its surface and is designed to serve as the negative control and is not responsive to any bacterial components.

NovýSCC635
≥1X10⁶ cells/vial   
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Replacement Information
Description
Catalogue NumberSCC635
DescriptionJurkat JE6.1 NF-kB::eGFP Cell Line
Alternate Names
  • Jurkat E6.1 NF-kB::eGFP
Background InformationContamination with bacterial components is a potential issue with the biologics for research- and therapeutic uses, as they can activate the so-called pattern-recognition receptors in mammalian cells and trigger immune responses, clouding the experimental results or even causing health hazards. Such responses are often mediated by the Toll-like Receptors (TLRs), which recognize bacterial molecular patterns to activate, among other things, cytokine expression through the NF-ĸB pathway.

The current assessment method for bacterial contamination measures lipopolysaccharide (LPS), the active component of endotoxins. Endotoxin measurement relies on its reaction with the limulus amoebocyte lysate (LAL), derived from the horseshoe crab blood.3 While the method is well established, however, the use of primary lysate obtained from wild animals presents a major sustainability issue.4 Recombinant alternatives to the primary LAL method have recently been gaining acceptance, but all LAL-based methods measure only the endotoxin contamination, and other classes of bacterial components go undetected.

To address these unmet needs, the junket JE6.1 cell line has been engineered to form a group of cell lines, each expressing a specific set of TLRs and harboring an eGFP expression cassette fused to the NF-ĸB Response Element. The result is that each of these strains expresses eGFP in response to a specific class of bacterial molecules.

SCC635, Jurkat JE6.1 NF-ĸB::eGFP cell line, expresses only the endogenous TLR6 on its surface and is designed to serve as the negative control for the group. It is not responsive to any bacterial components tested thus far, including the LPS, (ligands for SCC630, the TLR4 cell line), the Gram positive- and Gram negative flagellins (ligands for SCC631, the TLR5 cell line), and FSL-1 (ligand for SCC632, the TLR2/TLR6 cell line), and expresses eGFP only when the NF-ĸB Response Element is chemically activated. It has also been tested negative for PAM3CSK4 (ligand for TLR2/TLR1).

Source
SCC635 cell line was created by the deletion of the TLR5 gene in the Jurkat JE6.1 cell line harboring the eGFP expression cassette fused to the NF-kB Response Element.

References
1. Steinberger, et al. A Highly Sensitive Cell-Based TLR Reporter Platform for the Specific Detection of Bacterial TLR Ligands. Front Immunol. 2021; 12: 817604.
References
Product Information
Components
  • ≥1x106 viable Jurkat JE6.1 NF-kB::eGFP Cells: (Catalog No. SCC630). Store in liquid nitrogen.
Quality LevelMQ100
Applications
ApplicationJurkat JE6.1 NF-ĸB::eGFP cell line expresses only the endogenous TLR6 on its surface and is designed to serve as the negative control and is not responsive to any bacterial components.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
HostHuman
Cell Line Type
  • Immune
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage ConditionsJurkat JE6.1 NF-kB::eGFP cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
SCC635 04065271208629