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407673 Interleukin-8, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhIL-8

407673
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human interleukin-8 expressed in E. coli. A potent proinflammatory cytokine produced by monocytes, fibroblasts, and keratinocytes in response to stimulation by LPS, IL-1, or TNF-α, and by T lymphocytes in response to PHA stimulation. Induces adhesion of neutrophils to endothelial cells. A physiological initiator of chemotactic migration of leukocytes. May be involved in chronic inflammation. IL-8 acts as an angiogenic factor in human gastric carcinoma.
Catalogue Number407673
Brand Family Calbiochem®
SynonymsrhIL-8
References
ReferencesKido, S., et al. 2001. Eur. J. Cancer 37, 1482.
Wilson, A.J., et al. 1999. Clin. Sci. 97, 385.
Harada, A., et al. 1994. J. Leukoc. Biol. 56, 559.
Mazzucchelli, L., et al. 1994. Am. J. Pathol. 144, 997.
Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol. 9, 617.
Matsushima, K., et al. 1988. J. Exp. Med. 167, 1883.
Schröeder, J., et al. 1987. J. Immunol. 139, 3474.
Product Information
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 150-300 ng/ml as measured by induction of myeloperoxidase release from human neutrophils
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 EU/µg IL-8
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
407673 0

Documentation

Interleukin-8, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Interleukin-8, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407673

References

Přehled odkazů
Kido, S., et al. 2001. Eur. J. Cancer 37, 1482.
Wilson, A.J., et al. 1999. Clin. Sci. 97, 385.
Harada, A., et al. 1994. J. Leukoc. Biol. 56, 559.
Mazzucchelli, L., et al. 1994. Am. J. Pathol. 144, 997.
Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol. 9, 617.
Matsushima, K., et al. 1988. J. Exp. Med. 167, 1883.
Schröeder, J., et al. 1987. J. Immunol. 139, 3474.

Citations

Název
  • Felicity J. Ashcroft, et al. (2004) Control of expression of the lectin-like protein Reg-1 by gastrin: role of the Rho family GTPase RhoA and a C-rich promoter element. Biochemical Journal 381, 397-403.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision14-July-2008 RFH
    SynonymsrhIL-8
    DescriptionRecombinant, human interleukin-8 expressed in E. coli. IL-8 is produced by monocytes, fibroblasts, and keratinocytes in response to stimulation by LPS, IL-1, or TNFα, and by T lymphocytes in response to PHA stimulation. Induces adhesion of neutrophils to endothelial cells. May be involved in chronic inflammation.
    FormLyophilized
    Purity≥97% by SDS-PAGE
    ContaminantsEndotoxin: ≤100 EU/µg IL-8
    Biological activityED₅₀ = 150-300 ng/ml as measured by induction of myeloperoxidase release from human neutrophils
    SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
    Storage ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesKido, S., et al. 2001. Eur. J. Cancer 37, 1482.
    Wilson, A.J., et al. 1999. Clin. Sci. 97, 385.
    Harada, A., et al. 1994. J. Leukoc. Biol. 56, 559.
    Mazzucchelli, L., et al. 1994. Am. J. Pathol. 144, 997.
    Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol. 9, 617.
    Matsushima, K., et al. 1988. J. Exp. Med. 167, 1883.
    Schröeder, J., et al. 1987. J. Immunol. 139, 3474.
    Citation
  • Felicity J. Ashcroft, et al. (2004) Control of expression of the lectin-like protein Reg-1 by gastrin: role of the Rho family GTPase RhoA and a C-rich promoter element. Biochemical Journal 381, 397-403.
    		•