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AP202A Goat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate

Detect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Rat
AP202A
0.5 mL  
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Description
Catalogue NumberAP202A
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate
Background InformationLight chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain's mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
References
Product Information
FormatAlkaline Phosphatase
PresentationPurified by immunoaffinity chromatography. Liquid in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.
Quality LevelMQ100
Applications
ApplicationDetect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern Blotting: 1:5,000 -1:50,000

ELISA: 1:5,000 -1:50,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPrepared from purified rat IgG light chain.
EpitopeKappa light chain
HostGoat
SpecificityThe antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteMinimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.
Antibody TypePolyclonal Antibody
Purification MethodImmunoAffinity Purified
Molecular Weight25 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
Packaging Information
Material Size0.5 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
AP202A 04053252368073