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401432 Goat Anti-Human IgG, H & L Chain F(ab’)₂ Fragment Peroxidase Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
401432
  
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Replacement Information
Description
Overview

This product has been discontinued.





Adsorbed against bovine, horse, and mouse serum proteins.
Catalogue Number401432
Brand Family Calbiochem®
References
Product Information
FormLyophilized
FormulationLyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (1:5000-1:100,000)
Frozen Sections (1:500-1:5000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunocytochemistry (1:500-1:5000)
Paraffin Sections (1:500-1:5000)
Application CommentsBased on immunoelectrophoresis, this antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. Does not react with non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins. Antibody may, however, react with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenHuman IgG
ImmunogenHuman
HostGoat
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 500 µl dH₂O. Centrifuge if not completely clear after solubilizing for 2 h at room temperature. Following reconstitution, refrigerate (4°C). Undiluted stock solution is stable for up 6 weeks at 4°C. For long-term storage, add an equal volume of sterile glycerol and freeze (-20°C) (Note: addition of glycerol will decrease the protein and buffer concentration by half). Following the addition of glycerol, stock solution is stable for up to 1 year at -20°C. Diluted antibody is stable for up to 1 day at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
401432 0

Documentation

Goat Anti-Human IgG, H & L Chain F(ab’)₂ Fragment Peroxidase Conjugate Certificates of Analysis

TitleLot Number
401432
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationELISA (1:5000-1:100,000)
Frozen Sections (1:500-1:5000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunocytochemistry (1:500-1:5000)
Paraffin Sections (1:500-1:5000)
DescriptionImmunoaffinity purified goat polyclonal supplied as the F(ab')2 fragment conjugated to horseradish peroxidase. Recognizes human IgG, heavy and light chains.
HostGoat
Immunogen speciesHuman
ImmunogenHuman IgG
IsotypeIgG
Specieshuman
FormLyophilized
FormulationLyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
PreservativeNone
CommentsBased on immunoelectrophoresis, this antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. Does not react with non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins. Antibody may, however, react with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 500 µl dH₂O. Centrifuge if not completely clear after solubilizing for 2 h at room temperature. Following reconstitution, refrigerate (4°C). Undiluted stock solution is stable for up 6 weeks at 4°C. For long-term storage, add an equal volume of sterile glycerol and freeze (-20°C) (Note: addition of glycerol will decrease the protein and buffer concentration by half). Following the addition of glycerol, stock solution is stable for up to 1 year at -20°C. Diluted antibody is stable for up to 1 day at 4°C.
Toxicity Standard Handling