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71863 Gateway® Nova pET-60-DEST™ Expression System

71863
  
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Replacement Information
Description
Overview

This product has been discontinued.





The Gateway® Nova pET-60-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal GST•Tag™ coding sequence and a thrombin cleavage site. The GST•Tag enables gentle purification of fusion proteins using GST•Bind® resin. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal His•Tag® coding sequence. The presence of a "gentle elution" tag at each terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.
Catalogue Number71863
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
71863 0