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324762 Endopeptidase, Neutral, Porcine Kidney

Endopeptidase, Neutral, Porcine Kidney MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin

324762
  
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Replacement Information
Description
Overview

This product has been discontinued.



Native, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
Catalogue Number324762
Brand Family Calbiochem®
SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
References
ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
Product Information
Unit of DefinitionOne unit of enzyme will liberate 1.0 µmol <i>p</i>NA per min from Glutaryl-(Ala)₂-Phe-<i>p</i>NA at 25°C, pH 7.4.
EC number3.4.24.11
FormLyophilized
FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Specific Activity≥850 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
324762 0

Documentation

Endopeptidase, Neutral, Porcine Kidney MSDS

Title

Safety Data Sheet (SDS) 

Endopeptidase, Neutral, Porcine Kidney Certificates of Analysis

TitleLot Number
324762

References

Přehled odkazů
Barnes, K., et al. 1995. J. Neurochem. 64, 1826.
Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
DescriptionNative, neutral endopeptidase from porcine kidney. A cell surface zinc-containing proteinase that is abundant in the brush border membranes of the kidney proximal tubules. Also present in the brain, endothelial cells, liver, and the lung tissue. Peptidase activity is specifically directed towards the cleavage on the amino side of hydrophobic amino acid residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
FormLyophilized
FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
Recommended reaction conditionsSee Gafford, J. T., et al. 1983 (below)
EC number3.4.24.11
Purity≥95% by HPLC
Specific activity≥850 units/mg protein
Unit definitionOne unit of enzyme will liberate 1.0 µmol pNA per min from Glutaryl-(Ala)₂-Phe-pNA at 25°C, pH 7.4.
SolubilityPhysiological buffers, pH 7.4 (e.g., Tris-buffered saline) (1 mg/ml)
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
Toxicity Standard Handling
ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
Gafford, J.T., et al. 1983. Biochemistry 22, 3265.