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251755 DARPP-32, Rat, Recombinant, E. coli

DARPP-32, Rat, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dopamine- and cAMP-regulated Phosphoprotein

251755
  
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Přehled

Replacement Information
Description
OverviewRecombinant, rat DARPP-32 expressed in E. coli. DARPP-32 is an important component of dopaminergic function in the brain. Acts as an intracellular third messenger. Phosphorylation of DARPP-32 at Thr34 by cAMP-dependent protein kinase (PKA), results in its conversion to a potent inhibitor of protein phosphatase-1 (PP1; IC50 = 1-2 µM). Conversely, Thr34-phosphorylated DARPP-32 is dephosphorylated and inactivated in vitro by calcineurin (PP2B) and protein phosphatase-2A (PP2A). In resting neostratial slices, cyclin-dependent kinase 5 (Cdk5) phosphorylates DARPP-32 at Thr35 thereby reducing the efficacy of dopaminergic signaling.
M.W. 32,000 (by SDS-PAGE).
Catalogue Number251755
Brand Family Calbiochem®
SynonymsDopamine- and cAMP-regulated Phosphoprotein
References
ReferencesHiguchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.
Product Information
FormWhite lyophilized solid
Hygroscopic Hygroscopic
Applications
Biological Information
Primary TargetPP1
Primary Target IC<sub>50</sub>1-2 µM
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
251755 0

Documentation

DARPP-32, Rat, Recombinant, E. coli Certificates of Analysis

TitleLot Number
251755

References

Přehled odkazů
Higuchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.

Citations

Název
  • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision12-December-2008 RFH
    SynonymsDopamine- and cAMP-regulated Phosphoprotein
    DescriptionRecombinant, rat DARPP-32 expressed in E. coli. DARPP-32 (dopamine- and cAMP-regulated phosphoprotein) is an important component of dopaminergic function in the brain. Acts as an intracellular third messenger. Phosphorylation of DARPP-32 at Thr34 by cAMP-dependent protein kinase (PKA) converts it into a potent inhibitor of protein phosphatase 1 (IC50 = ~1 µM). However, phosphorylation at Thr35 by Cdk5 converts it into a competitive inhibitor of PKA. It is dephosphorylated and inactivated by calcineurin and protein phosphatase 2A (PP2A) in vitro. Also, found in the kidney where it participates in the regulation of the phosphorylation state of Na+, K+ -ATPase and control of salt balance. In adipocytes, it plays a role in adipogenesis and insulin signaling by maintaining low PP1 activity.
    FormWhite lyophilized solid
    Purity≥95% by SDS-PAGE
    Solubility50 mM Tris-HCl, pH 7.0
    Storage -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Standard Handling
    ReferencesHiguchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.
    Citation
  • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
    		•