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234155 Collagenase, Type II, Clostridium histolyticum

234155
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.



Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.

Catalogue Number234155
Brand Family Calbiochem®
References
ReferencesKeller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.
Product Information
CAS number9001-12-1
Activity≥200 collagenase units/mg dry weight
Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.
EC number3.4.24.3
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
ContaminantsNon-specific proteolytic activity: ≥50 fluorescence units/mg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 42/43-36/37/38

May cause sensitization by inhalation and skin contact.
Irritating to eyes, respiratory system and skin.
S PhraseS: 22-24-37

Do not breathe dust.
Avoid contact with skin.
Wear suitable gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
234155 0

Documentation

Collagenase, Type II, Clostridium histolyticum MSDS

Title

Safety Data Sheet (SDS) 

Collagenase, Type II, Clostridium histolyticum Certificates of Analysis

TitleLot Number
234155

References

Přehled odkazů
Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2010 RFH
DescriptionCrude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.
FormLyophilized
CAS number9001-12-1
EC number3.4.24.3
ContaminantsNon-specific proteolytic activity: ≥50 fluorescence units/mg
Activity≥200 collagenase units/mg dry weight
Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.
SolubilityAqueous Buffers
Storage Protect from moisture
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Toxicity Harmful
Merck USA index14, 2481
ReferencesKeller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.