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227037 Cholera Toxin, A Subunit, Vibrio cholerae, Type Inaba 569B

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
227037
  
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Description
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The subunit responsible for activation of adenylate cyclase. Binds to the ADP-ribosylation factor and modulates its activity. Catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit.
Catalogue Number227037
Brand Family Calbiochem®
References
ReferencesMcCloskey, M.A., and Zhang, L. 2000. J. Cell. Biol. 148, 137. Tsai, S.C., et al. 1988. J. Biol. Chem. 263, 1768. Van Heynigen, S. 1982. Biosci. Rep. 2, 135. Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552. Lai, C.Y., et al. 1976. J. Infect. Dis 139, 674. Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294. Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.
Product Information
ActivityThe activity of the intact toxin is greater than or equal to that of a standard cholera toxin preparation as determined in an ADP ribosylation assay. Each lot of purified A subunit is 2-3 times more active than the intact toxin as an activator of adenylate cyclase (weight basis). In an ADP-ribosylation assay, the specific enzymatic activity of a typical lot of A subunit is approximately 2.0 times that of cholera toxin on a weight basis.
DeclarationNot available for sale outside of the United States.
FormSolid
FormulationLyophilized from 200 mM NaCl, 50 mM Tris, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
Applications
Biological Information
Physicochemical Information
ContaminantsB subunit and intact toxin: none detected by native PAGE
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 21/22-36/37/38

Harmful in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 7-36/37

Keep container tightly closed.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C.
End use certificateY
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
227037 0

Documentation

Cholera Toxin, A Subunit, Vibrio cholerae, Type Inaba 569B Certificates of Analysis

TitleLot Number
227037

References

Přehled odkazů
McCloskey, M.A., and Zhang, L. 2000. J. Cell. Biol. 148, 137. Tsai, S.C., et al. 1988. J. Biol. Chem. 263, 1768. Van Heynigen, S. 1982. Biosci. Rep. 2, 135. Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552. Lai, C.Y., et al. 1976. J. Infect. Dis 139, 674. Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294. Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.

Brochure

Title
Activators and Inhibitors of Adenylate Cyclase Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2008 RFH
DescriptionCholera toxin is an oligomeric protein consisting of a single A subunit (M.W. 28,000) containing two polypeptide chains, A1 and A2, and a B subunit (M.W. 55,000) containing five polypeptide chains. Cholera toxin is the pathogenic agent responsible for the symptoms of cholera. Cholera toxin is also a potent activator of adenylate cyclase that binds the ADPribosylation factor and modulates its activity. The B subunit binds specifically to GM1 ganglioside receptors, which are expressed ubiquitously in mammalian cells, and assists in the transport of the A subunit through the membrane. The A subunit catalyzes the ADP-ribosylation of Gs which leads to the persistent activation of adenylate cyclase. The consequent increase in intracellular cAMP levels stimulates Cl- secretion and leads to severe dehydration. Cholera toxin is isolated from Vibrio cholerae type Inaba 569B by a modification of the methods of Rappaport, et al. and Mekalanos, et al. The purified A and B subunits are produced by a modification of the methods of Lai, et al.
FormSolid
FormulationLyophilized from 200 mM NaCl, 50 mM Tris, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
ContaminantsB subunit and intact toxin: none detected by native PAGE
ActivityThe activity of the intact toxin is greater than or equal to that of a standard cholera toxin preparation as determined in an ADP ribosylation assay. Each lot of purified A subunit is 2-3 times more active than the intact toxin as an activator of adenylate cyclase (weight basis). In an ADP-ribosylation assay, the specific enzymatic activity of a typical lot of A subunit is approximately 2.0 times that of cholera toxin on a weight basis.
SolubilityReconstitute with sterile distilled water. Please see vial label for reconstitution volume. Swirl gently during reconstitution as vigorous shaking or pipetting will result in damage to the toxin and a concomitant loss of biological activity. The purified A subunit is hydrophobic and vortexing may be required to get it into solution. DO NOT FREEZE solutions of cholera toxin subunits.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C.
Toxicity Highly Toxic
ReferencesMcCloskey, M.A., and Zhang, L. 2000. J. Cell. Biol. 148, 137. Tsai, S.C., et al. 1988. J. Biol. Chem. 263, 1768. Van Heynigen, S. 1982. Biosci. Rep. 2, 135. Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552. Lai, C.Y., et al. 1976. J. Infect. Dis 139, 674. Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294. Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.

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Kategorie

Life Science Research > Inhibitors and Biochemicals