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219498 Cathepsin S Substrate, Fluorogenic - Calbiochem

219498
  
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Description
Overview

This product has been discontinued.



An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (kcat/Km = 1.018 µM-1 S-1), but not by Cathepsin B & L (kcat/Km = 0.004 and 0.032 µM-1 S-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages. (excitation maximum: 340 nm; emission maximum: 405 nm)

Catalogue Number219498
Brand Family Calbiochem®
SynonymsMca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
References
ReferencesLutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.
Product Information
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₃H₁₂₃N₂₅O₂₃S
Chemical formulaC₉₃H₁₂₃N₂₅O₂₃S
Hygroscopic Hygroscopic
Quality LevelMQ100
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceMca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Katalogové číslo GTIN
219498 0