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218800 Caspase-7, Human, Recombinant, E. coli

Caspase-7, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mch3, ICE-LAP3

218800
  
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Přehled

Replacement Information
Description
OverviewRecombinant, human caspase-7 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.
M.W. 12,000 and 20,000 (heterodimer).
Catalogue Number218800
Brand Family Calbiochem®
SynonymsMch3, ICE-LAP3
References
ReferencesStennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=235400">Cat. No. 235400</a>) per hour at 37°C, pH 7.2.
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥15,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
218800 0

Documentation

Caspase-7, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Caspase-7, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218800

References

Přehled odkazů
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsMch3, ICE-LAP3
DescriptionRecombinant, human caspase-7 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.
FormLyophilized
Specific activity≥15,000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.
SolubilityReconstitute to 1 unit/µl in PBS, 15% glycerol.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesStennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.