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L6113-BC Biocoll

L6113-BC
  
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Replacement Information
Description
OverviewBiocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. Densities of up to 1.1 g/ml can be adjusted using this hydrophilic polymer. For optimal pH and osmolality, adjusting Biocoll with an acid, preferably amidotrizoeic acid(ATA), and sodium hydroxide is required. Biocoll with densities of 1.077 g/ml (cat. no. L 6113/5) and 1.090 g/ml (cat. no. L 6125) are already adjusted accordingly.

Specifications:

Sterility: Pass
Functional Test: Pass
pH: 6.37-7.57
Osmolality: 0.285-0.327 Osm/kg H20
Endotoxin: Pass
Density: 1.076-1.078 g/ml
Color: Colorless
Appearance: Clear

Separating solutions:

Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

Recommendations:

The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. One can also adjust a required density yourself diluting Easycoll correspondingly.

Separating lymphocytes using Biocoll:

1. Biocoll separating solution (D = 1.077 at +20°C) is given to 15 or 25 ml centrifugal tubes in volumes of 7 ml and 10 ml, resp.
2. Equal parts of heparinized whole blood (50 U/ml heparin, stabilizer-free) and culture medium are mixed and carefully applied over Biocoll separating solution.
3. Centrifuge 1200xg for 20 min.
4. Take layer of enriched (70 - 100 %) lymphocytes (between plasma and Biocoll) with a Pasteur′s pipette and wash twice in culture medium:
• for 10 min at 300xg
• for 10 min at 200xg
5. Cell count as usual.
Catalogue NumberL6113-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Storage +2°C to +25°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
L6113-BC 0

Documentation