Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

OP102 Anti-von Hippel-Lindau (VHL) Mouse mAb (Ig33)

OP102
  
Purchase on Sigma-Aldrich

Přehled

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~30 kDa von Hippel-Lindau (VHL) protein in prostate, lung, colon, breast, bladder or carcinoma tissue.
Catalogue NumberOP102
Brand Family Calbiochem®
References
ReferencesCorliss, C.L., et al. 1997.Human Pathology 28, 459.
Lee, S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1770.
Siemeister, G., et al. 1996. Cancer Res. 56, 2299.
Tsuchiya, H., et al. 1996. Cancer Res. 56, 2881.
Chen, F., et al. 1995. Cancer Res. 55, 4804.
Duan, D.R., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 6459.
Duan, D.R., et al. 1995. Science 269, 1402.
Iliopoulos, O., et al. 1995. Nat. Med. 1, 822.
Kishida, T., et al. 1995. Cancer Res. 55, 4544.
Kley, N., et al. 1995. Glia 15, 297.
Wizigmann-Voos, S., et al. 1995. Cancer Res. 55, 1358.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlProstate, kidney, lung, colon, breast, bladder, or thyroid carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Kibel, A., et al. 1995. Science 269, 1444.
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1-2 µg/ml)
Paraffin Sections (2-4 µg/ml; heat pre-treatment required, see comments)
Application CommentsPrior to staining formalin-fixed, paraffin-embedded tissue sections, boil the tissue in 1 mM EDTA, pH 8.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant fusion protein consisting of amino acids 1-213 of human VHL
ImmunogenHuman
CloneIg33
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Global Trade Item Number
Katalogové číslo GTIN
OP102 0