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07-2129 Anti-phospho-Abi-1 (Ser225) Antibody

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Anti-phospho-Abi-1 (Ser225) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ABI1, SSH3BP1 UniProt Number: Q8IZP0
07-2129
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, H, M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-2129
DescriptionAnti-phospho-Abi-1 (Ser225) Antibody
Alternate Names
  • Abl interactor 1
  • Abelson interactor 1
  • Abi-1
  • Abl-binding protein 4
  • AblBP4
  • Eps8 SH3 domain-binding protein
  • Eps8-binding protein
  • Nap1-binding protein
  • Nap1BP
  • Spectrin SH3 domain-binding protein 1
  • e3B1
Background InformationAbl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.
References
Product Information
FormatAffinity Purified
Control
  • EGF-treated COS cells untreated and treated with lambda phosphatase.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.
EpitopePhosphorylated Ser225
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Abi-1 phosphorylated at Ser225.
Species Reactivity
  • Monkey
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Monkey and Human.
Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol
  • ABI1
  • SSH3BP1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level (By similarity).

SUBUNIT STRUCTURE: Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1 (By similarity). Interacts with SOS1, SOS2, GRB2, SPTA1 and the first SH3 domain of NCK1. Isoform 6 does not interact with NCK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2.

SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus (By similarity). Cell projection › lamellipodium (By similarity). Cell projection › filopodium (By similarity). Cell projection › growth cone (By similarity). Cell junction › synapse › synaptosome (By similarity). Cytoplasm › cytoskeleton (By similarity). Note: Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes (By similarity).

TISSUE SPECIFICTY: Widely expressed, with highest expression in brain.

DOMAIN: The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A (By similarity).

PTM: In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.

INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.

SEQUENCE SIMILARITIES: Belongs to the ABI family.

Contains 1 SH3 domain.

Contains 1 t-SNARE coiled-coil homology domain.
Molecular Weight~60 kDa observed.
55 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
07-2129 04053252292774