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MABF2057-25UG Anti-minicore HCV Antibody, clone Neo4

Anti-minicore HCV, clone Neo4, Cat. No. MABF2057, is a mouse monoclonal antibody that detects HCV core and minicore proteins in HCV infected cells and has been tested for use in Immunofluorescence and Western Blotting.

Anti-minicore HCV Antibody, clone Neo4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology UniProt Number: Q9WMX2
MABF2057-25UG
25 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
VirusIF, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2057-25UG
DescriptionAnti-minicore HCV Antibody, clone Neo4
Alternate Names
  • HCV
  • Hepatitis C virus minocores
Background InformationHepatitis C virus (HCV) is a small (~55 65 nm) in size), enveloped, positive-sense single-stranded RNA virus that is a causative factor for hepatitis C and hepatocellular carcinoma. HCV expresses several proteins that promote viral replication. Genome polyprotein of HCV is a core protein that packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It also modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Envelope (E1 and E2) glycoproteins of HCV form a heterodimer, which is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. P7 protein, a heptameric ion channel protein, is essential for infectivity. HCV also contains NS2-3, which acts as a cysteine protease responsible for the autocatalytic cleavage of NS2-NS3. It undergoes self-inactivation following maturation. NS3 protein of HCV displays serine protease, NTPase, and RNA helicase activities. NS4B protein is shown to induce a specific membrane alteration that serves as a scaffold for the virus replication complex. NS5A protein is a component of the replication complex and is involved in RNA-binding. It is reported to mediate interferon resistance by interacting with and inhibiting human EIF2AK2/PKR. HCV also expresses proteins that are derived from the core gene and are known as minocores. These minicores lack the N-terminus, but contain the C-terminal portion of the mature p21 nucleocapsid protein. They are associated with higher risk of hepatocellular carcinoma, insulin resistance, and failure of interferon-based therapy.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-minicore HCV, clone Neo4, Cat. No. MABF2057, is a mouse monoclonal antibody that detects HCV core and minicore proteins in HCV infected cells and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected minicore HCV in Western Blotting applications (Eng, F.J., et. al. (2017). Hepatol Commun. 2(1):21-28).

Immunofluorescence Analysis: A representative lot detected minicore HCV in Immunofluorescence applications (Eng, F.J., et. al. (2017). Hepatol Commun. 2(1):21-28).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of Hepatitis C virus (HCV).
CloneNeo4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Neo4 detects HCV core and minicore proteins in cells infected with HCV virus.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 8 and 21 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in extracts of HEK293T cells transfected with plasmids expressing only HCV p21 core and 91 minicore proteins.

Western Blotting Analysis: 4 ug/mL of this antibody detected minicore HCV in extracts of HEK293T cells transfected with plasmids expressing only HCV p21 core and 91 minicore proteins.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF2057-25UG 04054839516856