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PC04 Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus

PC04
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~185 kDa c-ErbB2/c-Neu protein in Sk-BR-3 cells.
Catalogue NumberPC04
Brand Family Calbiochem®
SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
References
ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Negative controlNormal human fibroblasts
Positive controlSK-BR-3 cells
PreservativeNone
Applications
Application ReferencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
Application CommentsAntibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC04 0

Documentation

Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb Certificates of Analysis

TitleLot Number
PC04

References

Přehled odkazů
Di Fiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
ApplicationImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~185 kDa native and denatured forms of c-ErbB2/c-Neu.
BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification may be a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. Neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
IsotypeIgG
Specieshuman, rat
Positive controlSK-BR-3 cells
Negative controlNormal human fibroblasts
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual sample types.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Application referencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.