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ABN185 Anti-alpha B-crystallin Antibody

This Anti-alpha B-crystallin Antibody is validated for use in WB, IH(P) for the detection of alpha B-crystallin.

Anti-alpha B-crystallin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CRYAB, CRYA2 UniProt Number: P02511
ABN185
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, R, M, Ox, Eq, Rhesus MacaqueWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN185
DescriptionAnti-alpha B-crystallin Antibody
Alternate Names
  • Alpha-crystallin B chain
  • Alpha(B)-crystallin
  • Heat shock protein beta-5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component
Background InformationAlpha B-crystallin is a major structural protein in the vertebrate eye lens, but is also found in cardiac and skeletal muscles, kidney, and brain. Alpha B-crystallin is also found to be a component of Rosenthal fibers and a consistent tissue marker of epileptic foci. It occurs as large aggregates of up to 106 Da, composed of two proteins, αA- and αB-crystallin. The αA gene encodes a polypeptide of 173 residues, while αB-crystallin gene encodes a 175 residue polypeptide. αB-crystallin, like the small heat shock proteins (sHsp's), is induced by heat or osmotic shock and is upregulated by stress. α-crystallin and the sHsp's protect other proteins from heat-induced aggregation. The two other crystallin families, β and ɣ, are homologous to each other but not to the α-family or the sHsp's.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • Bovine calf lens, human kidney and calf kidney tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-alpha B-crystallin Antibody is validated for use in WB, IH(P) for the detection of alpha B-crystallin.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in rat lens fiber tissue. A 1:2,000 dilution from a representative lot detected Alpha-crystallin B chain in strong rat lens fiber tissue (data not provided). A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in strong muscle fiber tissue (data also not provided). It is recommended to use a 1:2,000 dilution only for strong rat lens fiber tissue.
Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of human kidney and calf kidney tissue lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus human alpha B-crystallin.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of alpha B-crystallin.
Species Reactivity
  • Bovine
  • Human
  • Rat
  • Mouse
  • Ox
  • Horse
  • Rhesus Macaque
Species Reactivity NoteDemonstrated to react with Human, Bovine, and Rat. Predicted to react with Mouse, Ox, Horse, and Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCrystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Gene Symbol
  • CRYAB
  • CRYA2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May contribute to the transparency and refractive index of the lens.

SUBUNIT STRUCTURE: Homodimer. Aggregates with homologous proteins, including CRYAA and the small heat shock protein HSPB1, to form large heteromeric complexes. Interacts with HSPBAP1 and TTN/titin.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Molecular Weight~20 kDa observed. Uncharacterized bands at ~37 and 43 kDa may be observed in some cell lyates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluted by Western Blot in bovine calf lens tissue lysate.

Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of bovine calf lens tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABN185 04053252756573

Documentation

Anti-alpha B-crystallin Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-alpha B-crystallin Antibody Certificates of Analysis

TitleLot Number
Anti-alpha B-crystallin - 2145716 2145716
Anti-alpha B-crystallin - 2458009 2458009
Anti-alpha B-crystallin - 2227169 2227169
Anti-alpha B-crystallin - 2298401 2298401
Anti-alpha B-crystallin - 3215523 3215523
Anti-alpha B-crystallin - 3255438 3255438
Anti-alpha B-crystallin - 3499363 3499363
Anti-alpha B-crystallin - 3722162 3722162
Anti-alpha B-crystallin - 3928028 3928028
Anti-alpha B-crystallin - 4132015 4132015
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