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MABC203 Anti-Yes-associated protein (YAP1) Antibody, clone 4D11.1

Anti-Yes-associated protein (YAP1) Antibody, clone 4D11.1 detects level of Yes-associated protein (YAP1) & has been published & validated for use in WB & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: YAP1, YAP65 UniProt Number: P46937
MABC203
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC203
DescriptionAnti-Yes-associated protein (YAP1) Antibody, clone 4D11.1
Alternate Names
  • Yorkie homolog
  • Yes-associated protein
  • Yap65
Background InformationThe YAP1 gene encodes a protein that binds to the SH3 (Src homology 3) domain of the Yes proto-oncogene product. As a transcriptional regulator, it can act as a coactivator and a corepressor, and serves as a critical downstream regulatory target in the Hippo signaling pathway. Additionally, it plays a critical role in regulating organ size and in tumor suppression by restricting proliferation and promoting apoptosis. The human YAP1 gene is well conserved among higher eukaryotes, but may not be conseved in yeast. Yes-associated protein expression is almost ubiquitous in adult human tissues, but is not detectable in peripheral blood leukocytes.
References
Product Information
FormatPurified
Control
  • Hela cell lysate
PresentationPurified mouse monoclonal IgG1κ supernatant containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Yes-associated protein (YAP1) Antibody, clone 4D11.1 detects level of Yes-associated protein (YAP1) & has been published & validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Yes-associated protein (YAP1) in HeLa and A431 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Yes-associated protein (YAP1).
Clone4D11.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
Gene Symbol
  • YAP1
  • YAP65
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

SUBUNIT STRUCTURE: Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2. Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif. The phosphorylated form interacts with YWHAB. Interacts (via WW domains) with LATS1 (via PPxY motif 2). Interacts with LATS2. Isoform 2 and isoform 3 interact (via WW domain 1) with isoform 3 of ERBB4 (via PPxY motif 2). Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Interacts with TP73. Interacts with RUNX1.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.

TISSUE SPECIFICITY: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Molecular Weight~65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Yes-associated protein (YAP1) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC203 04053252686832

Documentation

Anti-Yes-associated protein (YAP1) Antibody, clone 4D11.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Yes-associated protein (YAP1) Antibody, clone 4D11.1 Certificates of Analysis

TitleLot Number
Anti-Yes-associated protein (YAP1), clone 4D11.1 - 2201518 2201518
Anti-Yes-associated protein (YAP1), clone 4D11.1 - 2266777 2266777
Anti-Yes-associated protein (YAP1), clone 4D11.1 - 2309337 2309337
Anti-Yes-associated protein (YAP1), clone 4D11.1 - 3319401 3319401
Anti-Yes-associated protein (YAP1), clone 4D11.1 - 4091265 4091265
Anti-Yes-associated protein (YAP1), clone 4D11.1 - N1956542 N1956542
Anti-Yes-associated protein (YAP1), clone 4D11.1 -2794807 2794807
Anti-Yes-associated protein (YAP1), clone 4D11.1 Monoclonal Antibody 3112673

Brochure

Title
New Products: Volume 3, 2012