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ABS1676 Anti-VPS25

Anti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.

Anti-VPS25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: VPS25, DERP9, EAP20 UniProt Number: Q9BRG1
ABS1676
100 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, HIF, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1676
DescriptionAnti-VPS25
Alternate Names
  • Vacuolar protein-sorting-associated protein 25
  • hVps25
  • Dermal papilla-derived protein 9
  • ELL-associated protein of 20 kDa
  • ESCRT-II complex subunit VPS25
Background InformationVacuolar protein-sorting-associated protein 25 (UniProt: Q9BRG1; also known as hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25) is encoded by the VPS25 (also known as DERP9, EAP20) gene (Gene ID: 84313) in human. VPS25 is a component of the ESCRT-II complex (endosomal sorting complex required for transport II) that is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is diffusely distributed throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on co-expression with CHMP6. It is expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression have been reported in heart, skeletal muscle, brain and lung.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VPS25 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Western Blotting Analysis: A 1:500 & 1:1,000 dilution from a representative lot detected VPS25 in lysate of transfected HEK293T cells (Sandrin, V., et. al. (2013). Retrovirology. 10:104; Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
Biological Information
ImmunogenGST-tagged full length human recombinant VPS25 protein.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specifically detects Vacuolar protein-sorting-associated protein 25 (VPS25) in human and monkey cells.
IsotypeIgG
Species Reactivity
  • Monkey
  • Human
Species Reactivity NoteHuman, Monkey.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VPS25
  • DERP9
  • EAP20
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~20 kDa observed; 20.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VPS25 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS1676 04054839201394