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662106 Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-E1A/E1B, C-terminal

662106
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~117 kDa E1A and the ~110 kDa E1B ubiquitin activating enzymes.
Catalogue Number662106
Brand Family Calbiochem®
SynonymsAnti-E1A/E1B, C-terminal
References
ReferencesShang, F., et al. 1997. J. Biol. Chem. 272, 23086.
Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.
Product Information
FormLiquid
FormulationUndiluted serum.
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:500)
Application CommentsThe antibody specifically reacts with human E1A and E1B. Does not cross-react with other proteins by immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (KHVRALVLELCCNDESGEDVEVPYVRYTIR) corresponding to amino acids from a common C-terminal sequence of human E1A and E1B
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
662106 0

Documentation

Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb Certificates of Analysis

TitleLot Number
662106

References

Přehled odkazů
Shang, F., et al. 1997. J. Biol. Chem. 272, 23086.
Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Proteasomes Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2007 RFH
SynonymsAnti-E1A/E1B, C-terminal
ApplicationImmunoblotting (1:500)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~117 kDa E1A and the ~110 kDa E1B ubiquitin activating enzymes.
BackgroundUbiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin-activating enzyme exists as two isoforms: E1A (~117 kDa), localized predominantly in the nucleus, and E1B (~110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (KHVRALVLELCCNDESGEDVEVPYVRYTIR) corresponding to amino acids from a common C-terminal sequence of human E1A and E1B
IsotypeIgG
Specieshuman
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsThe antibody specifically reacts with human E1A and E1B. Does not cross-react with other proteins by immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesShang, F., et al. 1997. J. Biol. Chem. 272, 23086.
Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.