Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC1177 Anti-Stat3b Antibody, clone 516 G10H9

Anti-Stat3b Antibody, clone 516 G10H9 is an antibody against Stat3b for use in Western Blotting, Immunoprecipitation.

Anti-Stat3b Antibody, clone 516 G10H9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Transcription Factors Gene Symbol: STAT3, ADMIO, APRF, HIES UniProt Number: P40763-3
MABC1177
100 μg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1177
DescriptionAnti-Stat3b Antibody, clone 516 G10H9
Alternate Names
  • Signal transducer and activator of transcription 3b
  • Stat3b
  • Acute-phase response factor, spliced isoform 3
  • DNA-binding protein APRF, spliced isoform 3
Background InformationSignal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins (1, 2, 3, 4, 5A, 5B, 6) constitute a family of proteins that mediate cytokine and growth factor receptors-initiated signaling events. Stat3 is activated upon phosphorylation on Y705 by Jak or receptor tyrosine kinases, leading to Stat3 homodimerization, nuclear translocation, and target genes transcription. Constitutively tyrosine-phosphorylated Stat3 is implicated in the initiation and progression of many types of cancers, while Stat3 gene knockout in mice is lethal at the early embryonic stage. Two major alternatively spliced Stat3 isoforms exist, the C-terminal end 55-amino acid sequence (a.a. 716-770) in the canonical alpha isoform (UniProt P40763-1) is truncated and replaced with a 7-amino acid sequence in the 722-amino acid beta isoform (UniProt P40763-3). Tyrosine-phosphorylated Stat3β homodimers are more stable and less susceptible to dephosphorylation, bind DNA more avidly and exhibit markedly prolonged nuclear retention than pStat3α homodimers.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Stat3b Antibody, clone 516 G10H9 is an antibody against Stat3b for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected 0.3-100 ng of human Stat3β (a.a. 127–722), but not Stat3α (a.a. 127–770), GFP fusion protein (Courtesy of Dr. David J. Tweardy, Baylor College of Medicine, Houston, TX).
Western Blotting Analysis: A representative lot specifically detected Stat3β (a.a. 127–722) GFP fusion protein, but not Stat3α (a.a. 127–770) or Stat3βΔCT7 (a.a. 127–717) GFP fusion that lacks Stat3β C-terminal end 7-a.a. (CT7) sequence (Bharadwaj, U., et al. (2014). Cancers (Basel). 6(4):2012-2034).
Immunoprecipitation Analysis: A representative lot specifically immunoprecipitated human Stat3β (a.a. 127–722) GFP fusion protein, but not Stat3α (a.a. 127–770) GFP fusion protein, exogenously expressed in MEFs lacking endogenous murine Stat3 (Bharadwaj, U., et al. (2014). Cancers (Basel). 6(4):2012-2034).
Biological Information
ImmunogenLinear peptide containing human Stat3b C-terminal end 7 a.a. (CT7).
EpitopeWithin Stat3beta C-terminal end 7 a.a. (CT7).
Clone516 G10H9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 516 G10H9 detects the C-terminal end 7-a.a. (CT7) sequence present in human/mouse spliced isoform Stat3B (UniProt P40763-3/P42227-2), but not in human/mouse spliced isoform Stat3A (UniProt P40763-1/P42227-1) or human/mouse isoform Del-701 (UniProt P40763-2/P42227-3).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • STAT3
  • ADMIO
  • APRF
  • HIES
Purification MethodProtein G Purified
UniProt Number
Molecular Weight83.13 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvalusated by Western Blotting of recombinant human Stat3β GFP fusion protein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.5 µg of human Stat3β (a.a. 127–722) GFP fusion protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC1177 04055977349436