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565942 Anti-Sialyl Lewis a Mouse mAb (KM231)

This Anti-Sialyl Lewis a Mouse mAb (KM231) is validated for use in Adhesion Blockade, ELISA, Flow Cytometry, Immunoblotting, Paraffin Sections Radioimmunoassay for the detection of Sialyl Lewis a.

Anti-Sialyl Lewis a Mouse mAb (KM231) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Sialyl Lea

565942
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the SLea carbohydrate epitope on glycoproteins and glycolipids on cell membranes and in the cytoplasm of breast, colon, ovarian and stomach cancers, and in lung adenocarcinoma.
Catalogue Number565942
Brand Family Calbiochem®
SynonymsAnti-Sialyl Lea
References
ReferencesNagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.
Product Information
FormLiquid
FormulationIn PBS, protein stabilizer, pH 7.2.
Preservative≤0.1% sodium azide
Applications
Chyba v nastavení dat. Pagelet <collection_feature_application_id-AB> nenalezen.
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Paraffin Sections
Radioimmunoassay
Application NotesAdhesion Blockade (see comments)
ELISA (see comments)
Flow Cytometry (10 µg/ml)
Immunoblotting (see comments)
Paraffin Sections (5 µg/ml)
Radioimmunoassay (see comments)
Application CommentsReacts strongly with an antigen in cell membranes and cytoplasm of various cancerous tissues, including lung adeno-carcinomas, and cancers of the stomach, colon, breast, and ovary. Reacts weakly with various normal tissues, including stomach mucosa, bronchus, pancreatic acinar cells, germ cells, intestinal epithelial metaplasia, coated epithelium of the colon, and epithelia of the pancreatic duct, gall duct, and renal tubule. Does not react with fat cells, connective tissue, inflammatory cells, or red blood cells. The antibody has also been reported to work for immunoblotting, ELISA, RIA, and adhesion blocking. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman gastric cancer cells
ImmunogenHuman
CloneKM231
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
565942 0

Documentation

Anti-Sialyl Lewis a Mouse mAb (KM231) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Sialyl Lewis a Mouse mAb (KM231) Certificates of Analysis

TitleLot Number
565942

References

Přehled odkazů
Nagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2007 RFH
SynonymsAnti-Sialyl Lea
ApplicationAdhesion Blockade (see comments)
ELISA (see comments)
Flow Cytometry (10 µg/ml)
Immunoblotting (see comments)
Paraffin Sections (5 µg/ml)
Radioimmunoassay (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes the Sialyl Lewis a epitope on glycoproteins ≥1000 kDa.
BackgroundSialyl Lewis a [SLea; NeuAcα2,3Galβ1,3(Fucα1,4)GlcNAc] is a non-myeloid-specific tetrasaccharide that acts as a minimal ligand for E-selectin (ELAM; endothelial leukocyte adhesion molecule), an endothelial cell-surface glycoprotein that mediates recruitment of neutrophils and monocytes to sites of endothelial inflammation. This monoclonal antibody is specific for the SLea epitope found on various cell-surface glycoproteins and glycolipids.
HostMouse
Immunogen speciesHuman
Immunogenhuman gastric cancer cells
CloneKM231
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn PBS, protein stabilizer, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsReacts strongly with an antigen in cell membranes and cytoplasm of various cancerous tissues, including lung adeno-carcinomas, and cancers of the stomach, colon, breast, and ovary. Reacts weakly with various normal tissues, including stomach mucosa, bronchus, pancreatic acinar cells, germ cells, intestinal epithelial metaplasia, coated epithelium of the colon, and epithelia of the pancreatic duct, gall duct, and renal tubule. Does not react with fat cells, connective tissue, inflammatory cells, or red blood cells. The antibody has also been reported to work for immunoblotting, ELISA, RIA, and adhesion blocking. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
Toxicity Standard Handling
ReferencesNagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.