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MABE1033 Anti-SMARCB1 Antibody, clone 2C2

Anti-SMARCB1 Antibody, clone 2C2 is an antibody against SMARCB1 for use in Western Blotting and Immunocytochemistry.

Anti-SMARCB1 Antibody, clone 2C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: SMARCB1, BAF47, INI1, SNF5L1
UniProt Number: Q12824
MABE1033
100 µg  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1033
DescriptionAnti-SMARCB1 Antibody, clone 2C2
Alternate Names
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
  • BRG1-associated factor 47
  • BAF47
  • Integrase interactor 1 protein
  • SNF5 homolog
  • hSNF5
  • SMARCB1
Background InformationSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1), also known as BRG1-associated factor 47 (BAF47) or Integrase interactor 1 protein or SNF5 homolog (hSNF5), and encoded by the gene name SMARCB1 or BAF47, is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein has also been shown to bind to and enhance the DNA joining activity of HIV-1 integrase. It has been found to be a tumor suppressor, and the loss of SMARCB1 has been associated with malignant rhabdoid tumors. Tumor cells of synovial sarcoma seem to have a post transcriptional regulatory mechanism for SMARCB1.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SMARCB1 Antibody, clone 2C2 is an antibody against SMARCB1 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected SMARCB1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A representative lot detected SMARCB1 in HeLa cells (Harada, A., et al. (2014). Mono. Antibodies in Immuno. and Immunotherapy. 33:49-51).
Western Blotting Analysis: A representative lot detected SMARCB1 in HeLa cells (Harada, A., et al. (2014). Mono. Antibodies in Immuno. and Immunotherapy. 33:49-51).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human SMARCB1.
Clone2C2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMARCB1
  • BAF47
  • INI1
  • SNF5L1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 45 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SMARCB1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE1033 04055977135282

Documentation

Anti-SMARCB1 Antibody, clone 2C2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-SMARCB1 Antibody, clone 2C2 Certificates of Analysis

TitleLot Number
Anti-SMARCB1, clone 2C2 -Q2550841 Q2550841

References

Reference overviewPub Med ID
Monoclonal antibodies in immunodiagnosis and immunotherapy
Harada, A; Hayashi, M; Kuniyoshi, Y; Semba, Y; Sugahara, S; Tachibana, T; Ohkawa, Y; Fujita, M
Monoclonal antibodies in immunodiagnosis and immunotherapy  33  49-51  2014

Zobrazit abstrakt 		24555937 24555937

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.