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NR07 Anti-Ryanodine Receptor Mouse mAb (C3-33)

This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.

Anti-Ryanodine Receptor Mouse mAb (C3-33) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NR07
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Přehled

Tabulka spec. kláve

Species ReactivityHostAntibody Type
Am, Av, Ca, F, Rb, RMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
NR07-100UG Plastová ampulka 100 μg
Přidat k oblíbeným položkám
Description
OverviewRecognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.
Catalogue NumberNR07
Brand Family Calbiochem®
Application Data

Detection of rat ryanodine receptor by immunocytochemistry. Sample: Rat brain hippocampus. Primary antibody: Anti-Ryanodine Receptor Mouse mAb (C3-33) (Cat. No. NR07) (1:50). Detection: fluorescence.
References
ReferencesSitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
Meszaros, L.G., et al. 1993. Nature 364, 76.
Lai, F.A., et al. 1992. Biochem. J. 288, 553.
Lai, F.A., et al. 1989. Nature 331, 315.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRat brain hippocampus
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Lai, F.A., et al. 1992. Amer. J. Physiol. 263, C365. Frozen Sections Junker, J., et al. 1994. J. Biol. Chem. 269, 1627.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesFrozen Sections (1 µg/ml, see application references)
Immunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
Application CommentsA ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogencanine cardiac ryanodine receptor (RYR)
ImmunogenCanine
CloneC3-33
HostMouse
IsotypeIgG₁
Species Reactivity
  • Amphibian
  • Avian
  • Canine
  • Fish
  • Rabbit
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalogové číslo GTIN
NR07-100UG 04055977210002